IRF-4结合蛋白对口腔鳞癌细胞生长作用的机制研究  

Effects of IRF-4 binding protein( IBP) on proliferation of OSCC cells

作　　者：潘磊 简从相[2] 李卓[2] 黄晓山[3] 

机构地区：[1]解放军85医院口腔科,上海200050 [2]成都军区总医院附属口腔医院 [3]成都医学院第一附属医院口腔科

出　　处：《西南国防医药》2014年第5期468-470,共3页Medical Journal of National Defending Forces in Southwest China

基　　金：四川省教育厅科研资助项目(11ZB204)

摘　　要：目的观察IRF-4结合蛋白(IBP)促进口腔鳞癌(OSCC)细胞Tca8113生长的机制。方法利用实验室保存的口腔鳞癌细胞株Tca8113和先前建立的高表达IBP的口腔鳞癌细胞株Tca8113/IBP以及对照空质粒细胞株Tca8113/C1,通过流式细胞术,观察IBP过表达对Tca8113细胞周期的影响,并通过免疫印迹实验检测相关细胞周期蛋白的表达变化。结果流式细胞术显示IBP能缩短OSCC细胞的G1期,促进其进入S期,从而加快OSCC的增殖;并且Tca8113/IBP组细胞cyclin D1的表达高于Tca8113及Tca8113/C1组;而cyclin E和P27Kip1在各组细胞中的表达未见明显变化。结论 IBP可能通过调节cyclin D1的表达从而缩短OSCC细胞的G1期,促进其进入S期,并加速OSCC细胞的增殖。Objective To observe the potential effects of IRF-4 binding protein（ IBP） on the proliferation of OSCC cells.Methods FACS analysis assay was applied to investigate the effects of over-expressed IBP on the cell cycling of Tca8113,Tca8113 /C1 and Tca8113 /IBP,while Western blotting assay was applied to detect the protein expression in related cell cycles. Results FACS analysis assay showed that IBP shortened the G1 /S phase of OSCC and fastened its proliferation; the expression of cyclin D1 in Tca8113 /IBP was stronger than those in Tca8113 and Tca8113 /C1,while no significant differences were observed in the expression of cyclin E and P27^kip1. Conclusions IBP may shorten the G1 /S phase of OSCC and fasten its proliferation by regulating the expression of cyclin D1.

关 键 词：IRF-4结合蛋白 口腔鳞癌 增殖 CYCLIN D1 

分 类 号：R739.8[医药卫生—肿瘤]