非离子表面活性剂对生物丁醇发酵的影响  

Effect of non-ionic surfactants on butanol production with Clostridium acetobutylicum ATCC 824

作　　者：侯同刚[1] 张跃冬[1] 陈秀芳[1] 关静[1] 牟新东[1] 

机构地区：[1]中国科学院青岛生物能源与过程研究所中国科学院生物基材料重点实验室,山东青岛266101

出　　处：《生物工程学报》2014年第5期784-792,共9页Chinese Journal of Biotechnology

基　　金：山东省优秀中青年科学家科研奖励基金(No.BS2009ZZ001)资助~~

摘　　要：传统的丙酮-丁醇发酵的产物浓度过低(丁醇终浓度约为1.3 wt%),导致后期分离成本过高,从而影响了该过程的经济性,限制了其工业化进程。本文研究了高添加量的小分子非离子表面活性剂对生物丁醇发酵的影响。以吐温80为例,实验表明,当表面活性剂添加量超过其临界胶束浓度后,丁醇发酵的终浓度会随着表面活性剂添加量的增加而增加。当添加量达到5 wt%时,丁醇终浓度可以达到1.6 wt%,远高于该菌种的抑制浓度(0.8 wt%)。为阐明表面活性剂的作用机理,实验考察了吐温80对丁醇的增溶效应以及对发酵菌体表面亲疏水性的影响。结果表明,吐温80对丁醇的增溶效果很小,而对菌体表面的亲疏水性有较明显的影响。The low butanol concentration of acetone-butanol-ethanol fermentation causes uneconomical product recovery. In this work, the effect of small molecule non-ionic surfactants on butanol fermentation was evaluated, using laboratory stocks of Clostridium acetobutylicum ATCC 824. Non-ionic surfactants substantially increased butanol production when additive amount was higher than 1% （W/W）. Butanol concentration reached 16.9 g/L with 5% （W/W） Tween 80 and 100 g/L glucose in a 5 L fermenter. It was found that surfactants micelle solubilization capacity to butanol was very limited, indicating that butanol could hardly enter the surfactants micelle. Butanol production improvement was probably caused by cell surface hydrophobicity change due to surfactants adsorption.

关 键 词：表面活性剂 吐温80 生物丁醇 毒性 增溶 

分 类 号：TQ923[轻工技术与工程—发酵工程]