液质联用技术检测紫薯蓣中5种氨基甲酸酯农药的方法研究  

Simultaneous determination of carbamate pesticide residues in purple yam by high performance liquid chromatography-tandem mass spectrometry

作　　者：王泽锋[1] 石玲[1] 苏一兰[1] 闵勇[1] 

机构地区：[1]红河学院理学院,云南蒙自661199

出　　处：《食品工业科技》2014年第11期306-308,314,共4页Science and Technology of Food Industry

基　　金：云南省科技厅应用基础研究项目(2013FZ120)

摘　　要：通过超高液相色谱-串联质谱联用（LC-MS/MS）技术,建立了一种高灵敏度检测紫薯蓣中农药残留的方法。样品经环己烷-乙酸乙酯（1∶1,V/V）提取,全自动凝胶净化在线浓缩仪净化,然后采用LC-ESI（＋）-MS/MS测定,对液质分离条件进行优化。实验结果表明,5种氨基甲酸酯农药在1.0~50.0μg/L范围内线性关系良好,相关系数0.9983~0.9999;在2.0~10.0μg/kg浓度范围内,加样回收率82.05%~97.27%之间,相对标准偏差2.85%~6.63%。该方法具有快速、准确、简便、灵敏、净化效果好、人为误差小的特点。The high sensitive liquid chromatography-tandem mass spectrometry（LC-MS/MS） had been developed for the detection of 5 carbamate pesticides in purple yam.After extraction of the pesticides with cyclohexane and ethyl acetate（v/v = 1：1 ）,the extract was purified by automatic gel purification system using cyclohexane and ethyl acetate（v/v = 1：1）as mobile phase.Analysis was performed by liquid chromatography-electrospray ionization- tandem mass spectrometry operated in the multiple reaction monitoring（MRM）mode,and the separation conditions were optimized.The results showed that calibration curves were linear and covered from t.O to 50.OiJg/L, and the correlation coefficients was 0.9983-9.9999.The average recoveries, measured at 2.0 to 10.0μg/kg, were in the range 82.05% to 97.27% for all of the compounds tested with relative standard deviations were between 2.85% to 5.63%.The results indicated that the method had been demonstrated to be simple ,accurate, practical and high sensitive for the determination of carbamates residues in purple yam samples.

关 键 词：紫薯蓣 氨基甲酸酯类农药 快速高分离液质联用技术 

分 类 号：TS201.1[轻工技术与工程—食品科学]