大孔吸附树脂分离大豆乳清废液中低聚糖技术的研究  

Separation and Purification of Oligosaccharide from Soybean Processing Liquid Waste by Macroreticular Resin

作　　者：李书海[1] 陈洪生[1] 唐秋菊[1] 刁静静[2] 李娟[1] 朱红梅[1] 

机构地区：[1]黑龙江八一农垦大学食品学院,大庆163319 [2]黑龙江八一农垦大学国家杂粮工程技术研究中心

出　　处：《黑龙江八一农垦大学学报》2014年第3期49-52,共4页journal of heilongjiang bayi agricultural university

基　　金：黑龙江省大学生创新训练项目;黑龙江省农垦总局科技攻关项目(HNK12A-04-10)

摘　　要：试验研究了大孔吸附树脂对大豆乳清废液中低聚糖的分离纯化技术。以大豆低聚糖的分离度和总糖回收率为评价指标,对不同类型吸附树脂、不同洗脱流速、进料量、进料浓度和分离温度等因素进行分析,得出DSK530型树脂对大豆低聚糖的分离效果较好,且在流速2 mL·min-1、进料浓度15%、进料量15 mL条件下,大豆低聚糖的分离度达到0.55,总糖回收率达到90%以上。The objective of the present study was to investigate separation and purification technology of oligosaccharide from soybean processing liquid waste by macroreticular resin. the separation degree of soybean oligosaccharide and total sugar recovery rate as evaluation index,the factors were analyzed that oligosaccharide purification degree of different resins,desorption velocity,feeding amount,feeding concentration and separation temperature. The results indicated DSK530 type resin was the best effective separation material and the best separation condition was desorption velocity 2 mL＆#183;min-1,feeding concentration fifteen percent,feeding amount 15 mL and the resolution of soybean oligosaccharide could obtain 0.55 and total sugar recovery rate was over ninety percent.

关 键 词：大豆乳清废液 低聚糖 大孔吸附树脂 分离 

分 类 号：Q534[生物学—生物化学]