吉非替尼和NS398对前列腺癌PC-3M细胞增殖和侵袭力影响的研究  

Effects of gefitinib and NS-398 on the proliferation and invasion ability of prostate cancer cell line PC-3 in vitro

作　　者：朱佳庚[1] 吴宏飞[2] 林建中[2] 

机构地区：[1]南京医科大学附属南京医院泌尿外科,210006 [2]南京医科大学附属南京明基医院泌尿外科

出　　处：《中华临床医师杂志（电子版）》2014年第8期55-57,共3页Chinese Journal of Clinicians(Electronic Edition)

基　　金：南京市科技计划项目(20121088);南京市医学科技发展项目(QRX11277)

摘　　要：目的应用表皮生长因子受体(EGFR)特异性阻断剂吉非替尼(Gefitinib)和环氧化酶2(COX-2)特异性阻断剂NS398单独或联合作用于前列腺癌PC-3M细胞,观察对细胞增殖和侵袭能力的影响及可能机制研究。方法采用四甲基偶氮唑蓝法(MTT)和Transwell检测Gefitinib和NS398应用对细胞增殖和侵袭能力的影响,应用实时荧光定量聚合酶链反应(qRT-PCR)和Western blot法检测药物应用前后基质金属蛋白酶9(MMP-9)、表皮生长因子(VEGF)基因和蛋白表达水平的变化。结果 MTT结果显示Gefitinib或NS398都可抑制PC-3M细胞增殖(P<0.05),两者联合应用作用更明显(P<0.01),Transwell结果表明两种阻断剂都能在一定程度上抑制细胞侵袭能力(P<0.05),联合应用后抑制作用更显著(P<0.01),qRT-PCR和Western blot结果提示,联合应用Gefitinib和NS398对MMP-9、VEGF的抑制作用明显高于单独应用(P<0.01)。结论 Gefitinib和NS398联合应用可显著抑制PC-3M增殖和侵袭转移,可能与抑制VEGF和MMP-9表达有关。Objective To observe the effects and possible mechanism of epidermal growth factor receptor specific inhibitor gefitinib and cyclooxygenase-2 specific inhibitor NS-398 on the proliferation and invasion ability of prostate cancer cell line PC-3M in vitro. Methods Cell proliferation was assayed by using MTT method.The invasion ability was examined by Transwell assay. The mRNA and protein expression of MMP-9 and VEGF was detected by quantitative real-time reverse transcription-polymerase chain reaction（qRT-PCR）, and Western blotting respectively. Results MTT analyses revealed that gefitinib and NS-398 combination markedly induced decrease in cell viability compared to either drug（P〈0.05）. Additionally, the two drugs combination showed a greater suppression in the invasion ability （P〈0.01）. Also, we found that the combination induced more profound decrease in the expression level of MMP-9 and VEGF mRNA and protein （P〈0.01）. Conclusions The results suggest that gefitinib and NS-398 combination can significantly suppress PC-3M cells proliferation and invasion ability. VEGF and MMP-9 gene down-regulation may be involved in this progress.

关 键 词：前列腺肿瘤 受体 表皮生长因子 环氧化酶2 增殖 侵袭力 CYCLOOXYGENASE 2 

分 类 号：R737.25[医药卫生—肿瘤]