ISSR分子标记技术及其在茶树研究中的应用  

Research and application of ISSR molecular markers in Camellia sinensis

作　　者：林志坤[1] 孙威江[1,2] 陈志丹[2] 林伟东[1] 肖思[1] 

机构地区：[1]福建农林大学园艺学院,福建福州350002 [2]福建农林大学安溪茶学院,福建泉州362400

出　　处：《广东农业科学》2014年第9期139-142,146,共5页Guangdong Agricultural Sciences

基　　金：国家"十二五"农村领域国家科技计划项目(2013AA10260605);国家"十二五"科技支撑计划项目(2011BAD01B01)

摘　　要：随着现代生物技术的发展,DNA分子标记技术在茶树研究领域的应用越来越多,ISSR分子标记技术以其高效、快速、稳定、可靠等诸多优点而被广泛应用。介绍了ISSR分子标记在构建植物DNA指纹图谱上的应用,综述了ISSR分子标记技术在茶树亲缘关系研究、遗传多样性分析、品种鉴定、遗传图谱构建等领域中的应用研究进展,探讨了ISSR分子标记在茶树研究中存在的一些问题,为更好地应用ISSR技术开展构建茶树DNA指纹图谱等研究提供参考。With the development of modern biotechnology, DNA molecular marker technologies cue widely applied in tea plant. ISSR molecular marker technique is efficient, fast, stable and reliable. Several molecular marker techniques and their applications in plant were introduced. The application and research progresses of ISSR melecular marker in genetic relationship, genetic diversity, varieties identification and genetic mapping of tea plant were summarized. The problems of ISSR molecular marker technique in tea plant and its application prospects in the establishment of tea DNA fingerprinting were also analyzed in order to provide better reference for DNA fingerprint establishment of tea plant based on ISSR molecular marker technique.

关 键 词：ISSR分子标记 DNA指纹图谱 茶树 种质资源 

分 类 号：S571.1[农业科学—茶叶生产加工]