miR-146a基因多态性与腔隙性脑梗死的关系  

Association between miR-146a gene polymorphism and lacunar infarction

作　　者：李友[1] 朱菁[1] 马国达[1] 李克深[1] 崔理立[1] 

机构地区：[1]广东医学院附属医院,广东湛江524001

出　　处：《山东医药》2014年第16期1-3,共3页Shandong Medical Journal

基　　金：国家自然科学基金资助项目(81300929)

摘　　要：目的探讨miR-146a基因多态性与腔隙性脑梗死(LI)的关系。方法选择LI患者173例(观察组)和与其性别、年龄匹配的非心脑血管疾病体检者298例(对照组),采用SNaPshot法检测血miR-146a rs2910164和rs57095329位点的基因多态性,B型彩色多普勒超声仪检测颈动脉内膜中层厚度(CIMT)。结果观察组miR-146a rs2910164位点GG基因型频率为8.67%、CC+CG基因型频率为91.33%、G等位基因频率为33.24%,对照组分别为17.11%、82.89%、39.93%,P均<0.05。两组miR-146a rs57095329基因型和等位基因频率比较,P均>0.05。观察组70岁以下者miR-146a rs2910164位点G等位基因频率为28.57%,对照组为39.12%,P<0.05。观察组miR-146a rs2910164和rs57095329位点正常和突变基因型患者的CIMT比较,P均>0.05。结论 LI患者miR-146a基因rs2910164位点G等位基因频率降低,G等位基因可能是其发病的一种保护性因素。Objective To investigate the association between miR-146a gene polymorphism and lacunar infarction （ LI） .Methods The present study recruited 173 patients with LI （ observation group ） and 298 age-and sex-matched out-patient controls without cardiovascular and cerebrovascular diseases （ control group ） .Multiplex SNaPshot was employed to determine the genotype and allele frequencies of rs 2910164 and rs57095329 polymorphisms of the miR-146a gene.B-color Doppler ultrasonography was used to determine the carotid artery intima-media thickness （ CIMT） .Results The frequen-cies of the GG genotype , CC＋CG genotype and G allele in the observation group were 8.67%, 91.33%and 33.24%, re-spectively;While the frequencies in the control group were 17.11%, 82.89%and 39.93%, respectively （all P〈0.05）. The genotype and allele frequencies of rs57095329 were not statistically associated with LI （all P〉0.05）.The frequency in patients below 70 years old of the observation group carrying the G allele of miR-146a rs2910164 polymorphism was 28.57%, and the frequency in the control group was 39.12% （P〈0.05）.The CIMT of common genotype and mutant genotype between rs2910164 and rs57095329 polymorphisms of miR-146a in the observation group was not significantly dif-ferent （all P〉0.05）.Conclusion The present study suggests that the frequency of the G allele of miR-146a rs2910164 is lower in LI patients and the rs 2910164 G allele is likely to play a protective role in the etiology of lacunar infarction .

关 键 词：脑小血管病 腔隙性脑梗死 基因多态性 微小RNA-146a 

分 类 号：R743.3[医药卫生—神经病学与精神病学]