木糖醇对黏性放线菌生长及产酸影响的体外研究  

In vitro study of xylitol on the growth and acid production of Actinomyces viscosus

作　　者：郭厚佐 肖遥[1] 廉小天[1] 邹玲[1] 

机构地区：[1]口腔疾病研究国家重点实验室华西口腔医院(四川大学),成都610041

出　　处：《华西口腔医学杂志》2014年第3期217-220,共4页West China Journal of Stomatology

基　　金：四川大学口腔疾病研究国家重点实验室大学生训练计划基金资助项目(2012年)

摘　　要：目的对比不同质量浓度下木糖醇对黏性放线菌生长及产酸的影响。方法分别用含不同质量浓度(128、64、32、16、8、4 g·L-1)木糖醇的脑心浸液(BHI)液体培养基在厌氧条件下培养黏性放线菌,测定其最小抑菌质量浓度(MIC);然后测量对照组以及1/2、1/4、1/8 MIC和MIC质量浓度时培养1.5、3、6、12、24、48 h液体培养基的pH值,计算ΔpH值,同时测量2、4、6、8、10、12 h液体培养基的光密度(OD550)值;最后测定抑制50%及90%黏性放线菌生物膜形成的最低木糖醇质量浓度(即MBIC50和MBIC90)。运用SPSS 19.0进行数据分析。结果木糖醇能抑制黏性放线菌的生长,MIC为64 g·L-1。培养12 h后,各组之间的ΔpH值差异均有统计学意义(P<0.05),且ΔpH值随MIC质量浓度的增大而减小;此时,除1/2 MIC以及MIC组之外,其余各组的OD550差异均无统计学意义(P>0.05),OD550值随MIC质量浓度的增加而减小。提示黏性放线菌在含1/2 MIC及MIC的木糖醇培养基里生长及产酸能力随木糖醇质量浓度的升高而下降。木糖醇的MIBC50为64 g·L-1,MIBC90为128 g·L-1,说明木糖醇培养基能抑制黏性放线菌生物膜的形成。结论木糖醇能有效地抑制黏性放线菌的生长、黏附以及产酸,有一定的抑制致龋菌和防治龋病的效果。Objective This research aimed to study the inhibitory effect of xylitol on the growth and acid production of Actinomyces viscosus （A. viscosus）. Methods We cultivated A. viscosus in anaerobic conditions with different concentrations （128, 64, 32, 16, 8, and 4 g·L-1） of xylitol brain heart infusion liquid medium and determined the minimum inhibitory concentration （MIC）. Subsequently, we measured the pH value of the control group, as well as those of 1/2, 1/4, 1/8 MIC, and MIC concentration groups at 1.5, 3, 6, 12, 24, and 48 h. The ΔpH and OD550 at 2, 4, 6, 8, 10, and 12 h were calculated. We discovered that the minimum xylitol concentrations suppressed 50% and 90% A. viscosus biofilm formation （i.e., MBIC50 and MBIC90）. SPSS 19.0 was used to analyze the collected data, and conclusions were drawn afterward. Results Xylitol inhibited the growth of A. viscosus at MIC of 64 g·L-1. After 12 h, the differences of pH value among groups were all statistically significant （P〈0.05）, and ΔpH increased when the MIC concentration decreased. Except for the1/2 MIC and MIC groups, the differences of OD550 among groups had no statistical significance （P〉0.05）, and OD550 also increased when the MIC concentration decreased. These results imply that the ability of A. viscosus to grow and produce acid in 1/2 MIC and MIC conditions will be reduced with the increase in xylitol concentration. The value of MIBC50 was 64 g·L-1, whereas the value of MIBC90 was 128 g·L-1. This finding indicates that the xylitol medium can restrict A. viscosus biofilm formation. Conclusion Xylitolcan effectively inhibit the growth, adhesion, and acid production of A. viscosus, protecting teeth from cariogenic bacteria and preventing caries to a certain extent.

关 键 词：木糖醇 黏性放线菌 生长 产酸 

分 类 号：R781[医药卫生—口腔医学]