硒化乳酸菌胞外多糖对体外培养小鼠脾淋巴细胞分泌NO、IL-4及IL-12的影响  

Effect of Selenium Lactic Acid Bacteria Exopolysaccharides on Expression of NO,IL-4 and IL-12 in Mouse Spleen Lymphocyte

作　　者：刘鹭[1] 潘道东[1,2] 曾小群[1] 郭宇星[2] 

机构地区：[1]宁波大学海洋学院食品系,浙江宁波3152112 [2]南京师范大学食品系,南京210097

出　　处：《中国食品学报》2014年第3期17-21,共5页Journal of Chinese Institute Of Food Science and Technology

基　　金：浙江省自然基金重点项目(No.Z3110211);国家自然科学基金项目(No.41276121);宁波市重大攻关项目(No.2011C11017)

摘　　要：目的:研究硒化乳酸菌胞外多糖对体外培养小鼠脾淋巴细胞分泌NO,IL-4及IL-12的影响。方法:分离制备了小鼠脾淋巴细胞,用Griess法检测NO含量,双抗体夹心酶联免疫法检测IL-4和IL-12含量。结果:当多糖质量浓度为400μg/mL时,硒化EPS对ConA刺激的T淋巴细胞NO分泌量高于EPS。培养4 h后上清液中NO浓度显著升高,培养8 h达到最高。硒化EPS可抑制小鼠脾淋巴细胞分泌IL-4,400μg/mL硒化EPS对于ConA刺激的小鼠T淋巴细胞IL-12生成量影响最为显著。结论:硒化EPS在一定程度上影响小鼠脾淋巴细胞免疫功能,这可能是其免疫调控机理之一。Objective： The effect of NO,IL-4 and IL-12 by selenium exopolysaccharides（EPS） produced by lactic acid bacteria were investigated in this study.Method： The concentration of NO in supernatant of vitro Spleen Lymphocyte was tested by griess assay,and the concentration of IL-4 and IL-12 was determined by Elisa.Results： It showed that 400 μg/mL Se-EPS group contains higher concentration of NO in supernatant of lymphocyte activated by ConA compared to other groups.After 4 hours,the concentration of NO in supernatant became much higher.The maximum NO production were observed after 8 hours.Selenium EPS can significantly inhibited the production of IL-4.A maximum IL-12 production were observed in lymphocyte activated by conA when the concentration of added selenium EPS in culture solution was 400 μg/mL.Conclusion： Se-EPS could effect the immune function of mouse spleen lymphocyte,which may be one of mechanisms.

关 键 词：乳酸菌胞外多糖 硒化 淋巴细胞 NO 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]