正交试验优化猪血制备亚硝基血红蛋白  

An Optimized Method for Preparing Nitrosohemoglobin from Pig Blood

作　　者：程燕 姜无边 符绍辉 

机构地区：[1]湖北宝迪农业科技有限公司,湖北安陆432602 [2]天津宝迪农业科技股份有限公司,天津301800

出　　处：《肉类研究》2014年第3期10-13,共4页Meat Research

摘　　要：以猪血为原料,经过血细胞分离、破裂提纯得到血红蛋白,与NaNO2反应制取亚硝基血红蛋白,并采用正交试验法讨论NaNO2添加量、VC添加量、pH值、加热时间对合成试验结果的影响,以OD值评价试验结果。该方法是一种低成本、高效益的方法。结果显示:血红蛋白、NaNO2、VC物质的量比l∶2∶6、pH6.0、加热至60℃保持30 min为亚硝基血红蛋白合成试验的最佳工艺条件,可简单而快速的制得亚硝基血红蛋白。Hemoglobin was extracted from the ruptured red blood cells from pigs and then allowed to react with sodium nitrite to form nitrosohemoglobin. The efficiency of nitrosohemoglobin synthesis as evaluated by OD57s om was investigated with respect to four reaction conditions including the amounts of sodium nitrite and vitamin C, pH and heating time using an orthogonal array design. As a result, a simple, rapid and cost-effective method for preparing nitrosohemoglobin was established by heating a mixture of hemoglobin, sodium nitrite and vitamin C at a molar ratio of 1：2：6 adjusted to pH 6.0 to 60 ℃ and maintaining the temperature for 30 minutes.

关 键 词：猪血 血红蛋白 NaNO2 VC 亚硝基血红蛋白 

分 类 号：TS251.93[轻工技术与工程—农产品加工及贮藏工程]