细菌纤维素产生菌双向凝胶电泳分析技术  

Two-dimensional Polyacrylamide Gel Electrophoresis Techniques for Proteomic Research of Gluconacetobacter

作　　者：杨暄[1] 李俊[1] 李从发[1] 郝俊冉[2] 许文涛[2] 刘四新[1] 

机构地区：[1]海南大学食品学院,海南海口570228 [2]中国农业大学食品科学与营养工程学院,北京100083

出　　处：《热带生物学报》2014年第1期73-77,91,共6页Journal of Tropical Biology

基　　金：公益性行业(农业)科研专项(200903026-6)

摘　　要：对细菌纤维素产生菌株——葡糖酸醋杆菌菌体蛋白的提取过程、等电点的分布及纯化方法等方面进行了探索和优化.结果表明：葡糖酸醋杆菌蛋白主要分布在pI 4 ～7的范围内；采用超滤浓缩后进行2DClean-up试剂盒纯化,可以获得更优的蛋白分离效果；在考马斯亮蓝G250染色条件下,有效分离出（1037±65）个蛋白点；建立了高质量葡糖酸醋杆菌蛋白的双向凝胶电泳分析技术.The protein extraction,isoelectric point of distribution and protein purification of the proteins of Gluconacetobacter,a stain produced bacterial cellulose,were tried and optimized to establish a set of two-dimensional polyacrylamide gel electrophoresis （2DE） techniques for proteomic analysis of Gluconacetobacter.The results showed that Gluconacetobacter proteins were mainly distributed in the range of pI 4-7.The 2D Clean-up kit purification after ultrafiltration concentration gave better protein separation effect.Under Coomassie brilliant blue G250 condition,（1037 ±65） protein points were isolated.

关 键 词：细菌纤维素 欧罗巴葡糖酸醋杆菌 双向电泳 蛋白质组学 

分 类 号：Q815[生物学—生物工程]