香菇孢子单核体原生质体的制备和再生的研究  

Study on Preparation and Regeneration of Protoplast From Sporulated Monokaryon of Lentinus edodes

作　　者：鲍大鹏 谭琦[2] 吴国富 潘迎捷[2] 

机构地区：[1]安徽凤阳农业技术师范学院,安徽凤阳233100 [2]上海农业科学院食用菌研究所,上海201106

出　　处：《中国食用菌》2000年第6期3-5,共3页Edible Fungi of China

摘　　要：本研究以香菇四种交配型的孢子单核体为材料 ,用酶解的方法制备出高得率的具四种交配型的原生质体 ,且均可再生。研究表明 :四种交配型原生质体的再生率大小 ,与原生质体的得率高低并不是一致的 ,但与菌丝生长速度、再生菌落出现的时间以及交配型之间有一定的关系。这为原生质体的再生能力是受交配型基因控制的推断提供了实验证据。Four mating type protoplasts which all could regenerate were highly yielded with four mating type sporulated monokaryon of Lentinus edodes using enzyme digestion.The research indicated the regeneration rates of protoplasts didn′t tend to the output of protoplasts,and were effected by the hypha growing rate,occurring time of regeneration colony and mating type.The study also provided experiment proof for the inference in which the regeneration capability was controlled by the mating type gene.A great number of monokaryon protoplasts with single mating type could become a new material for fungi genetics study.

关 键 词：香菇 孢子单核体 原生质体 再生 制备 交配型 遗传变异 

分 类 号：S646.120.3[农业科学—蔬菜学]