胶红酵母JB401降解脱色三苯甲烷类染料  

Biodegradation and decolorization of triphenylmethane dye by Rhodotorula mucilaginosa JB401

作　　者：李国辉[1,2] 高剑平[3] 丁重阳[1,2] 刘元法[4] 张梁[1,2] 顾正华[2] 石贵阳[1,2] 章克昌[1] 

机构地区：[1]江南大学生物工程学院工业生物技术教育部重点实验室,无锡214122 [2]江南大学粮食发酵工艺与技术国家工程实验室,无锡214122 [3]漳州职业技术学院,漳州363000 [4]江南大学食品学院,无锡214122

出　　处：《生物加工过程》2014年第3期26-31,共6页Chinese Journal of Bioprocess Engineering

基　　金：江苏省高校优势资源学科建设工程

摘　　要：从烟梗中分离筛选得到1株能够对三苯甲烷类染料高效脱色的微生物,经ITS-5.8S rDNA分析鉴定为胶红酵母,命名为Rhodotorula mucilaginosa JB401。全波长扫描实验结果证实染料的脱色由胶红酵母降解结晶紫引起。为了提高R.mucilaginosa JB401脱色结晶紫的能力,通过单因素试验对R.mucilaginosa JB401的培养条件进行了优化,得出菌体生长24 h后以2%接种量接入初始pH为5的脱色培养基并在37℃摇床培养,可以取得最优脱色效果,此时脱色50、100和200 mg/L的结晶紫达到90%去除率分别需要3、6和14 h。此外,胶红酵母对温度和pH良好的适应性使其具有应用于工业废水处理的潜力。A strain efficiently decolorizing triphenylmethane dye was isolated from tobacco stem.By the analysis of ITS-5.8S rDNA,the strain was identified as Rhodotorula mucilaginosa JB401.The result of full wavelength scan confirmed that the delorization of crystal violet was caused by the dye degradation.In order to improve the the decolorization of crystal violet by R.mucilaginosa JB401,a single factor experiment was used to optimize the culture conditions of R.mucilaginosa JB401.The optimized decolorization conditions were 37℃,initial pH 5,2%inoculums under the shake-flask condition after cultivation for 24 h.Under the optimized culture conditions,50,100 and 200 mg/L of crystal violet were decolorized with 90%and time for decolorization were 3 h（50 mg/L）,6 h（100 mg/L） and 14 h（200 mg/L）, respectively.The excellent adaptability of R.mucilaginosa JB401 to pH and temperature exhibited potential applications.

关 键 词：生物降解 脱色 胶红酵母 三苯甲烷染料 

分 类 号：TQ926[轻工技术与工程—发酵工程] X79[环境科学与工程—环境工程]