食品和饲料中转基因植物FMV35S启动子环介导等温扩增检测方法的建立  

Establishment of a Method to Detect Genetically Modified Plant FMV35S Promoter in Food and Feeds Using Loop-mediated Isothermal Amplification

作　　者：邝筱珊[1] 胡松楠[1] 游淑珠[1] 成晓维[1] 王小玉[1] 冯家望[1] 

机构地区：[1]珠海出入境检验检疫局技术中心,广东珠海519015

出　　处：《安徽农业科学》2014年第16期4999-5001,5017,共4页Journal of Anhui Agricultural Sciences

摘　　要：[目的]建立FMV35S基因启动子环介导等温扩增的检测方法。[方法]建立一种检测食品和饲料中FMV35S转基因成分的环介导恒温扩增(LAMP)检测方法,并利用实时浊度法对该LAMP检测方法进行灵敏度、特异性和稳定性评价。[结果]该方法的检测限为0.5%,特异性和稳定性良好。采用LAMP法与荧光PCR方法进行了实际样品对比检测,结果一致可靠。[结论]可采用LAMP法对食品和饲料中FMV35S转基因成分进行检测。[Objective] To establish a method to detect genetically modified plant FMV35S promoter in food and feeds using loop-mediated isothermal amplification.[Method] A method was established to detect FMV35S gene in food and feed using loop-mediated isothermal amplification （LAMP）.The stability,specificity and sensitivity of the LAMP assay was evaluate by real-time turbidity method.[Result] The results showed that the concentration which LAMP assay can detect is as low as 0.5％ （W/W）,and the stability and specificity of the test is satisfied.[Conclusion] Comparing with the testing results between the real-time methods and LAMP method,it is confirmed that the method could be used to analyze FMV35S genetically modified ingredients in food or feed.

关 键 词：食品 饲料 FMV35S基因 环介导恒温扩增 检测 

分 类 号：S188[农业科学—农业基础科学]