绿茶提取物对人肝癌细胞株SMMC-7721体外凋亡的影响  

Effect of Epigallocatechin Gallate Extracted from Green Tea on the Apoptosis of Human Hepatocellular Carcinoma Cell Line SMMC-7721 in vitro

作　　者：蒲霞[1] 郭庆喜[1] 任美萍[2] 杨成万[1] 龙汉安[1] 

机构地区：[1]泸州医学院病理教研室 [2]泸州医学院药理学教研室泸州646000

出　　处：《天然产物研究与开发》2014年第5期770-773,786,共5页Natural Product Research and Development

基　　金：四川省教育厅科研项目基金(2008806)

摘　　要：从茶多酚中分离提纯表没食子儿茶素没食子酸酯(Epigallocatechin gallate,EGCG)单体。体外培养人肝癌SMMC-7721细胞,采用不同浓度的EGCG对其进行干预,用流式细胞仪检测其对肝癌细胞凋亡的影响;免疫细胞化学法和RT-PCR检测EGCG作用于SMMC-7721细胞后对Survivin、突变型P53 mRNA和蛋白表达的影响。结果显示在EGCG的作用下,SMMC-7721凋亡明显增加(P<0.05);Survivin和突变型P53蛋白和mRNA表达水平均下调(P<0.05)。表明EGCG可能通过下调人肝癌细胞株Survivin和突变型p53的表达,进而促进人肝癌细胞株SMMC-7721凋亡,以达到抗癌作用。In this study, epigaUocatechin gallate （EGCG） was isolated and purified from green tea polyphenols. The effect of EGCG on cultured human hepatoma SMMC-7721 cells was investigated. The apoptosis of cultured SMMC-7721 cells in different concentrations of EGCG were detected using flow cytometry. The expressions of mRNA and protein of Survivin,P53 in SMMC-7721 cells treated with EGCG were detected by reverse transcfiption-pelymerase chain reaction （RT-PCR） and immunocytochemistry technique, respectively. The results showed that after treating with EGCG, the ap- optosis of SMMC-7721 cells significantly raised （P 〈 0.05） ;the expression levels of protein and mRNA of Survivin and P53 were down-regulated （P 〈 0.05 ）. These results indicated that the antineoplastic effect of EGCG was possibly induced through directly or indirectly inhibiting expressions of Survivin and P53.

关 键 词：表没食子儿茶素没食子酸酯 肝癌细胞 凋亡 SURVIVIN 突变型P53 

分 类 号：R735.7[医药卫生—肿瘤] S571.1[医药卫生—临床医学]