茶多酚对人结肠癌细胞株Caco-2凋亡及RhoA蛋白活性的影响  

Influence of tea polyphenols on the apoptosis of human colon cancer cell line Caco-2 and RhoA protein activity

作　　者：董丽华[1] 朱旭 姜仕柱[1] 李琪毅[1] 

机构地区：[1]三峡大学仁和医院消化内科,湖北宜昌443001 [2]重庆市渝北区人民医院外二科,重庆401120

出　　处：《实用临床医药杂志》2014年第7期7-10,共4页Journal of Clinical Medicine in Practice

基　　金：三峡大学青年科学基金项目(KJ2011A021);中国高校医学期刊临床专项资金(11321250)

摘　　要：目的探讨茶多酚对人结肠癌细胞株Caco-2凋亡及RhoA蛋白活性的影响。方法将传代后的人结肠癌细胞株Caco-2细胞分为实验组和对照组,实验组加入不同浓度的茶多酚(25、50、100和200μmol/L),对照组加入等量RPMI-1640培养液,采用四甲基偶氮唑盐(MTT)法测定茶多酚对Caco-2增殖抑制的影响,流式细胞术(FCM)检测细胞凋亡指数,Pull-down法检测人结肠癌细胞株Caco-2细胞内的RhoA蛋白活性。结果不同浓度的茶多酚对人结肠癌细胞株Caco-2有明显的抑制作用,且呈剂量及时间依赖性;Pull-down法检测结果显示,茶多酚可以降低人结肠癌细胞株Caco-2细胞内的RhoA蛋白活性,且随浓度增加其蛋白活性降低。结论茶多酚可促进人结肠癌细胞株Caco-2的凋亡,其可能通过降低RhoA蛋白活性来实现。Objective To explore the influence of tea polyphenols （TP） on the apoptosis of human colon cancer cell line Caco-2 and RhoA protein activity. Methods The passage human colon cancer cell line Caco-2 ceils were divided into experimental group and control group. The experimental was added with different concentrations of TP （25, 50, 100 and 200 μmol/L） , while the control group was added with equal amount of culture fluid RPMI-1640. Inhibition of Caco-2 proliferation, ap- optosis indexes and RhoA protein activity within human colon cancer cell line Caco-2 cells were re- spectively detected by methyl thiazolyl tetrazolium （MTT）, flow cytometry （FCM） and Pull-down method. Results Different concentrations of TP showed significant inhibitory effects on human colon cancer cell line Caco-2. Pull-down test results showed that TP could reduce the activity of RhoA pro- tein in human colon cancer cell line Caco-2 cells, and with the increasing of concentration, its protein activity decreased. Conclusion TP can promote the apoptosis of human colon cancer cell line Caco-2, which may be achieved by reducing the activity of RhoA protein.

关 键 词：茶多酚 结肠癌 CACO-2 RHOA蛋白 

分 类 号：R735.3[医药卫生—肿瘤]