重组腺病毒bcl-xs基因对人卵巢癌裸鼠移植瘤作用的研究  

Therapeutic effects of adenovirus bcl-xs gene to the ascites tumor of nude mice model of human ovarian carcinoma

作　　者：陈爱平[1] 彭芝兰[1] 王和[1] 高国兰[1] 刘珊玲[1] Baker VV 

机构地区：[1]华西医科大学第二附属医院妇产科 [2]Comprehensive Cancer Centre,University of Michigen

出　　处：《中华妇产科杂志》2001年第3期165-167,共3页Chinese Journal of Obstetrics and Gynecology

基　　金：国家自然科学基金!资助项目 ( 39470 72 1)

摘　　要：目的　观察重组腺病毒bcl xs基因 (adv bcl xs基因 ,简称bcl xs基因 )对人卵巢癌裸鼠移植瘤的生长抑制和荷瘤裸鼠生存率的影响 ,为卵巢癌的基因治疗提供实验基础。方法　采用以复制缺陷型腺病毒bcl xs基因感染的人胚肾细胞 ,使bcl xs基因在人胚肾细胞内扩增 ,扩增后的bcl xs基因感染大鼠卵巢癌细胞株NUTU 19,观察bcl xs基因对NUTU 19细胞的生长抑制作用并检测其病毒滴度后 ,将bcl xs基因导入人卵巢癌裸鼠移植瘤中 ,观察bcl xs基因对腹水的生长抑制作用 ,计算裸鼠的生存时间 ,并观察bcl xs基因对裸鼠全身和局部的毒副作用 ;电镜观察bcl xs基因的病毒颗粒 ,以免疫细胞化学染色测定bcl xs基因表达情况。结果　bcl xs基因可在人胚肾细胞内扩增 ,对NUTU 19细胞有抑制和杀伤作用 ,并在裸鼠体内呈过度表达 ,使裸鼠腹水形成的时间由 5～ 10d延长至 11～ 2 3d ,裸鼠平均生存时间由 2 4d延长至 39d。bcl xs基因对裸鼠全身和局部无明显毒副作用。结论　bcl xs基因导入人卵巢癌裸鼠移植肿瘤 ,能延缓腹水形成 ,延长裸鼠的生存时间 。Objective To investigate the effects of adenovirus bcl xs gene on the ascites tumor growth inhibition and survival rate of nude mice with human ovarian carcinoma transplanted intraperitoneally Methods Making an adenovirus bcl xs gene vector infected in JH293T cell and reproduced in it After having detected the inhibitory potential of adenovirus bcl xs gene on NUTU 19 cell we use it to transfer intraperitoneally to ascites tumor model of human ovarian carcinoma transplanted in nude mice Detected the ascites formation, the survival time and survival rate of nude mice with the human ascites tumour The weight and toxic adverse systemically effects of nude mice was observed and morphology of adenovirus was observed by electromicroscope and the gene expression was detected by immunocellchemistry Results The adenovirus bcl xs gene could reproduce in JH293T cell and had inhibitory potential on NUTU 19 cell the survival time of nude mice was longer and the survival rate was higher, and the time of ascites formation was retarted There was no obvious alternation in the weight and systemic toxic adverse effects observed Conclusions The data suggestes that the transfer of adenovirus bcl xs gene to the ascites tumour of nude mice with human ovarian carcinoma could improve the survival rate of nude mice and retard the time of ascites formation It may be a useful method of gene therapy in the treatment of ovarian carcinoma

关 键 词：腺病毒科 癌基因 卵巢肿瘤 Ehrlich瘤 实验性肿瘤 bcl-xs基因 

分 类 号：R737.31[医药卫生—肿瘤]