IL-27在小鼠实验性肠炎模型中的表达  

Expression of Interleukin-27 in Mouse TNBS-Induced Experimental Colitis

作　　者：赵娇[1] 李冬青[2] 李一鸣[1] 刘娟[1] 叶梅[1] 

机构地区：[1]武汉大学中南医院综合科消化病区,湖北武汉430071 [2]武汉大学基础医学院微生物教研室,湖北武汉430071

出　　处：《武汉大学学报（医学版）》2014年第3期353-356,361,共5页Medical Journal of Wuhan University

基　　金：国家自然科学基金资助项目(编号:81270468);湖北省自然科学基金资助项目(编号:2011CDB530)

摘　　要：目的:通过检测IL-27在小鼠实验性肠炎模型中的表达,探讨其在肠炎发病机制中的作用。方法:建立三硝基苯磺酸(TNBS)诱导的急性小鼠实验性肠炎模型,采用实时荧光定量PCR(qRT-PCR)及Western blot分别检测IL-27基因mRNA和蛋白在炎症肠黏膜中的表达。结果:TNBS成功诱导急性小鼠实验性肠炎,与对照组相比,TNBS处理的小鼠炎症评分明显增高,炎症肠黏膜中IL-27mRNA和蛋白表达亦明显增高,差异具有统计学意义。结论:IL-27在TNBS诱导的小鼠炎症肠黏膜中表达明显增高,提示IL-27可能参与小鼠肠道炎症的形成过程。Objective： To explore the pathophysiological role of IL-27 in mouse experimental colitis, we investigated the expression of IL-27 in colitis of mice. Methods： We built the acute TNBS-induced colitis, and the mRNA and protein expression of IL-27 in the inflammatory mucosa of bowel was measured by real-time PCR and Western blot, respectively. Results= The mRNA and protein ex- pression of IL-27 in the inflammatory mucosa was significantly higher than that respectively in non-inflammation mucosa. Conclusion. The results suggest the IL-27 up-regulates in the colon in- flammation and is possible involved in the pathogenesis of colitis.

关 键 词：炎症性肠病 IL-27 T细胞 肠炎模型 

分 类 号：R574[医药卫生—消化系统]