大孔吸附树脂纯化生姜提取物中6-姜酚工艺优化  

Purification of 6-gingerol from Oleoresin with Macroporous Resin

作　　者：刘伟[1] 周春丽[1,2] 赵婧[1] 陈冬[1] 李全宏[1] 

机构地区：[1]中国农业大学食品科学与营养工程学院,北京100083 [2]江西科技师范学院生命科学学院,南昌330013

出　　处：《农业机械学报》2014年第6期237-241,328,共6页Transactions of the Chinese Society for Agricultural Machinery

基　　金：国家重点基础研究发展计划(973计划)资助项目(2012CB720805);'十二五'国家科技支撑计划资助项目(2011BAD23B01)

摘　　要：比较了6种大孔吸附树脂对6-姜酚的吸附和解吸性能,在静态吸附研究基础上筛选出效果较好的树脂进行动态试验,并通过HPLC-UV和GC-MS对纯化前、后的成分进行了定性和定量分析。结果表明:D101型大孔树脂更适合纯化姜油树脂中的6-姜酚,其纯化的最佳工艺条件为:上样液质量浓度2 mg/mL,流速2 mL/min,3 BV去离子水洗脱,然后用体积分数80%乙醇以2 mL/min的流速洗脱4 BV,可以使6-姜酚的纯度从1.54%提高到71.32%。In order to separate and purify 6-gingerol from oleoresin by macroporous resin, 6 kinds of macroporous resins were chosen to carry out static and dynamic adsorption-desorption experiments. Qualitative and quantitative analysis of purification results were analyzed by HPLC UV and GC - MS. The results showed that D101 was the optimal macroporous resin for separating and purifying 6-gingerol, the best technology parameters were as follows： sample concentration of 2 mg/mL, distilled water of 3 BV, 80% ethanol for elution velocity of 2 mL/min, elution volume of 4BV. Under these conditions, the purity of 6-gignerol enhanced from 1.54% to 71.32%

关 键 词：6-姜酚 大孔树脂 纯化 

分 类 号：TS201.1[轻工技术与工程—食品科学]