茶黄素对高糖诱导的人晶状体上皮细胞氧化损伤的影响  

Effect of Theaflavins on Human Lens Epithelial Cells in High Glucose Induced Oxidative Damage

作　　者：牛丽[1,2] 曹佩琴[1,2] 刘仲华[1,3,2] 

机构地区：[1]国家植物功能成分利用工程技术研究中心,长沙410128 [2]湖南农业大学植物资源工程系,长沙410128 [3]湖南省植物功能成分利用协同创新中心,长沙410128

出　　处：《茶叶通讯》2014年第1期15-17,23,共4页Journal of Tea Communication

摘　　要：研究茶黄素(Theaflavin,TF)对高糖(High glucose,HG)诱导的人晶状体上皮细胞(Human lens epithelial cells,HLECs)氧化损伤的影响。采用体外培养人晶状体上皮细胞,用葡萄糖诱导细胞成氧化损伤模型,外加一定浓度的茶黄素干预,比色法检测细胞体内SOD、CAT、GSH-Px、MDA活力和含量的变化。结果表明相对于正常培养的细胞,高糖诱导细胞体内SOD、CAT、GSH的活性降低,MDA含量上升;加入茶黄素后,提高了细胞内SOD、CAT、GSH-Px的活力,降低了细胞内MDA的含量,与模型组细胞相比,具有显著差异(P<0.01)。To study the effect of theaflavins on oxidative damage in human lens epithelial cells under high glucose. Methods：Human lens epithelial cells were cultured with HG （30mM）to Establish the oxidative damage model. After the culture under suitable temperature for 24h ,32μM and 64μM TF were added into TF-growps. The SOD、CAT、GSH-Px、MDA were measured with using colorimetry. Results：Compared with the normal cultured cells, The SOD, CAT, GSH-Px activity were redused in cells induced by high glucose, the MDA content increased;After add the TF, it improved the SOD, CAT, GSH activity, MDA content decreased（P〈0.01）. Conclusion： Theaflavins can effectively control the activities of antioxidant enzymes in human lens epithelial cells induced by high glucose .

关 键 词：茶黄素 高糖 人晶状体上皮细胞 氧化损伤 

分 类 号：Q255[生物学—细胞生物学]