Wnt-5a在原发性肝细胞癌发生中的表达及临床意义  

Expression and clinical significance of Wnt-5a gene in primary hepatocellular carcinomas

作　　者：李培峰[1] 刘晓红[1] 曹永成[1] 王翠翠[1] 周露婷[1] 耿明[1] 

机构地区：[1]济南军区总医院病理科,济南250031

出　　处：《中国医师杂志》2014年第5期588-591,共4页Journal of Chinese Physician

基　　金：国家自然科学基金（81172261）

摘　　要：目的 检测Wnt-5a基因和基因产物在原发性肝细胞癌中的表达,探讨Wnt-5a基因在肝癌发生过程中的作用和临床意义.方法 采用实时定量PCR技术,检测Wnt-5a mRNA在26例新鲜肝细胞癌和癌旁组织中的含量;用免疫组化法观察Wnt-5a蛋白在85例肝细胞癌和癌旁组织、15例肝硬化组织中的表达.结果 Wnt-5a mRNA在肝癌及癌旁组织中的相对含量分别为0.102 127±0.158 620和0.020 106±0.022 075,两者间差异有统计学意义（P＜0.05）;Wnt-5a蛋白在肝细胞癌、癌旁组织和肝硬化中的阳性率分别为21.2％ （18/85）、81.26％ （69/85）和86.7％ （13/15）,Wnt-5a在肝癌组织中的阳性表达显著低于肝硬化和癌旁组织,差异有统计学意义（P ＜0.01）;Wnt-5a蛋白在肝癌中的表达与肿瘤的TNM分期和血清甲胎蛋白（AFP）含量有关（P＜0.05）.结论 Wnt-5a基因在HCC基因转录中呈上调表达,而在蛋白水平显示为缺失和下调表达,提示肿瘤细胞是在蛋白翻译水平而不是转录水平干扰了Wnt-5a基因功能.Objective To investigate the expression of Wnt-5a gene in primary hepatocellular carcinoma （HCC） and to expose its role and clinical significance in the development of HCC.Methods Real time quantitative reverse transcription polymerase chain reaction （RT-PCR） was performed in 26 fresh HCC samples and the corresponding para-carcinoma tissues to detect mRNA expression of Wnt-5a gene.Wnt-5a protein was detected with immunohistochemical method in paraffin embedding tissues of 85 cases of HCCs and the corresponding para-carcinoma tissues,and 15 cases of hepatic cirrhosis.Results RT-PCR analysis showed that Wnt-5a mRNA （0.102 127 ±0.158 620） in the HCC tissues was more than that （0.020 106 ±0.022 075） in the para-carcinoma tissues （P〈0.05）.The positive expression rate of Wnt-5a protein in HCC,para-carcinoma,and hepatic cirrhosis tissues were 21.2% （18/85）,81.26% （69/85）,and 86.7% （13/15）,respectively.The positive rate of Wnt-5a was significantly lower in the HCC than in the para-carcinoma and hepatic cirrhosis tissues （P 〈 0.01）.The expression of Wnt-5a was significantly associated with lower tumor node metastasis （TNM） stages and small alpha fetoproteins （AFP） content of blood serum （P 〈0.05）.Conclusions The high expression of Wnt-5a mRNA was found in the gene transcription of HCC,while Wnt-5a protein was absent or low in HCC.It was suggested that the roles of Wnt-5a was interfered at the protein level rather than the transcriptional level in the HCC.

关 键 词：癌 肝细胞 遗传学 原癌基因 聚合酶链反应 免疫组织化学 

分 类 号：R735.7[医药卫生—肿瘤]