我国近海贝类体内帕金虫的亲缘关系  

Genetic relationship between Perkinsusin China's offshore shellfish

作　　者：周广麒[1] 刘雁[1] 李冬梅[2] 李秋菊[2] 刘沙[2] 梁玉波[2] 

机构地区：[1]大连工业大学生物工程学院,辽宁大连116034 [2]国家海洋环境监测中心,辽宁大连116023

出　　处：《大连工业大学学报》2014年第3期161-165,共5页Journal of Dalian Polytechnic University

基　　金：国家自然科学基金资助项目(30070124;30470275)

摘　　要：研究了我国近海贝类体内帕金虫的分布和分类。从辽宁、河北到广西、海南沿海九省区58个地点共30种主要的经济贝类采样分析,利用聚合酶链式反应(PCR)法,对样品进行rDNA-ITS序列的扩增,初步确定我国海产贝类体内帕金虫的种类及其分布。结果表明,我国具有两种rDNA-ITS序列差异较大的帕金虫,在菲律宾蛤仔、紫贻贝、毛蚶、皱纹盘鲍、牡蛎中检测出帕金虫Perkinsus olseni;在南方近江牡蛎中检测出帕金虫Perkinsus beihaiensis。The distribution and classification of Perkinsus spp.in China＇s offshore shellfish were studied.Thirty different kinds of major economic shellfish from fifty-eight locations in nine coastal provinces of China from north to south such as Liaoning,Hebei,Guangxi,Hainan,was sampled and analyzed.PCR was applied to amplify the rDNA-ITS sequences,and thus the species and their distribution of Perkinsus spp.was preliminarily identified.The results indicated that China has two different kinds of Perkinsus spp.and the differences in rDNA-ITS sequences are large.These two different Perkinsus sp.are respectively P.olseni detected fromRuditapes philippinarum,Mytilus, Scapharca subcrenata,Haliotis discus hannai and Oysters,and P.beihaisens detected from Crassostrea rivularis living in Southern China.

关 键 词：贝类 帕金虫 PCR RDNA-ITS 

分 类 号：TS254.2[轻工技术与工程—水产品加工及贮藏工程] S944.4[轻工技术与工程—食品科学与工程]