UPLC-MS-MS测定玉米制品中4种黄曲霉毒素的检测方法研究  

Determination method of 4 aflatoxins in corns products by UPLC-MS-MS

作　　者：马彦杰[1] 曾凡刚[1] 董彬[2] 

机构地区：[1]中国人民大学环境学院,北京100872 [2]河北省疾病预防控制中心,石家庄050000

出　　处：《食品科技》2014年第6期307-310,共4页Food Science and Technology

基　　金：北京市自然科学基金项目(8132040)

摘　　要：建立了检测玉米中4种黄曲霉毒素(AFs)的超高效液相色谱-串联质谱(UPLC-MS-MS)检测方法。称取5.00 g玉米制品样品后加入甲醇-水溶液(v:v=7:3)提取,经过多功能净化柱净化,然后进入UPLC-MS-MS进行检测。4种AFs的保留时间小于3 min,无需衍生化过程。最小检测限分别为AFB1:0.05μg/kg,AFB2:0.02μg/kg,AFG1:0.09μg/kg,AFG2:0.04μg/kg,线性相关系数(r2)均大于0.99,回收率在81.5%～108.8%之间,RSD在2.9%～12.9%之间。此方法具有良好的精密度和准确度,适用于玉米制品中AFs的检测。对市场上采购的3种玉米制品进行检测,发现普遍含量较低,在0.093～7.3μg/kg之间。对不同地区的玉米制品样品检测发现,该方法适用于玉米制品的快速灵敏检测要求。An ultra high performance liquid chromatography-tandem mass spectrometry （UPLC-MS-MS） method was developed to simultaneous determination of 4 aflatoxins （AFs） in corns. The samples were extracted with methanol samples-water （v：v=7：3） and purificated by multifunction purification column after weighed. Then the samples were detected by UPLC-MS-MS. The retention time is less than 3 minutes without derivatization. The minimum detection limits were 0.05 μg/kg, 0.02 μg/kg, 0.09 μg/kg and 0.04 μg/kg. The linear correlation coefficient （r^2） was greater than 0.99 and the average recovery ratios were 81.5%-108.8%, the RSD were 2.9%-12.9%. This method had good precision and accuracy for the detection of AFs in corn products. Generally, the AFs were at a low level between 0.093-7.3 μg/kg. The detection of corn products for different regions shows that this method is suitable for rapid and sensitive detection.

关 键 词：黄曲霉毒素(AFs) 玉米制品 超高效液相色谱-串联质谱法(UPLC-MS—MS) 

分 类 号：TS207.5[轻工技术与工程—食品科学]