人亮氨酸拉链肿瘤抑制基因1在肾癌组织的表达及意义  

Transcription level and significance of leucine-zipper tumor suppressor 1 in renal cell carcinoma

作　　者：顾朝辉[1] 田凤艳[1] 贾占奎[1] 金志波[1] 孟政雷 李冠儒[1] 孙科[1] 魏金星[1] 杨锦建[1] 阚全程[2] 

机构地区：[1]郑州大学第一附属医院泌尿外科、河南省泌尿外科研究所、郑州市泌尿外科肿瘤分子生物学重点实验室,450052 [2]郑州大学第一附属医院药学部,450052

出　　处：《中华实验外科杂志》2014年第6期1193-1195,共3页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目（81100464、81200883）;中国博士后科学基金资助项目（2012M521410）;河南省卫生厅科技攻关计划资助项目（2011020039）

摘　　要：目的探讨人亮氨酸拉链肿瘤抑制基因1（LZTS1）在肾癌组织中的表达及意义。方法收集28例肾癌标本及正常肾组织。采用逆转录-聚合酶链反应（RT—PCR）、实时定量逆转录聚合酶链反应（RT—qPCR）和原位杂交方法检测LZTS1在肾癌和正常肾组织中的表达水平。结果常规RT-PCR和RT—qPCR显示肿瘤组LZTS1 mRNA相对表达量（0．39±0．04，n=28）较正常组织组LZTS1 mRNA相对表达量（1．40±0．06，n=28）低，两组间差异有统计学意义（P〈0．05）。原位杂交结果显示，正常肾组织中LZTS1 mRNA表达丰度较大，采用Image—Pro软件分析其组织表达量，正常肾组织组阳性率为92．9％（26／28，n=28），肿瘤组阳性率为14．2％（4／28，n=28），两组间差异有统计学意义（P〈0．05）。结论LZTS1在肾癌组织中表达缺失，可能参与肾癌的分子发生过程。Objective To detect the expression level of leucine-zipper tumor suppressor 1 （ LZTS1 ） in normal renal tissues and renal cell carcinoma tissues and evaluate its significance. Methods Twenty-eight cases of normal renal tissues and renal cell carcinoma tissues were harvested. The expression level of IZTS1 mRNA was detected by reverse transeriptaseolymerase chain reaction （RT-PCR） , realtime reverse transcriptase-polymerase chain reaction （RT-qPCR） and in situ hybridization （ISH）. Results The relative level of LZTS1 mRNA in renal tumor group was （ 0. 39 ± 0. 04, n = 28 ） by RT-qPCR via 2 - A AC, method. Meanwhile, the relative level of LZTSI mRNA in normal renal tissue group was （ 1.40 ± 0. 06, n = 28 ）. The expression level of LZTS1 mRNA in renal tumor tissue was downregulated as compared with that in normal renal tissue with the difference being significant （ P 〈 0. 05）. The in situ hybridization showed only less brown precipitate in renal tumor tissue than in normal renal tissue. The positive expression rate of LZTS1 mRNA was 92. 9% （26/28,n =28） in normal renal tissue, and that was 14. 2% （4/28, n = 28 ） in renal tumor tissue. The results of ISH were consistent to the PCR resuhs. Conclusion The loss of LZTS1 maybe participate in the molecular process of carcinogenesis in renal cell carcinoma.

关 键 词：亮氨酸拉链肿瘤抑制基因1 肾癌 

分 类 号：R737.11[医药卫生—肿瘤]