兔抗人ERA多克隆抗体的纯化  

Purification of rabbit anti-human ERA polyclonal antibody

作　　者：万亚坤[1] 张俊杰[1] 陈南春[1] 陈苏民[1] 

机构地区：[1]第四军医大学生化与分子生物学教研室

出　　处：《细胞与分子免疫学杂志》2001年第2期166-168,共3页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金资助No.39870380;全军医药卫生科研基金资助;No.98M108

摘　　要：目的纯化兔抗人ERA的多克隆抗体。方法在大肠杆菌中,表达重组MBPhEra融合蛋白。表达产物先继以直链淀粉树脂亲和柱和Superose12凝胶柱过滤纯化。将纯化的MBPhEra偶联于NHSactivatedSepharoseTM上,制备亲和层析柱,纯化兔抗人ERA多克隆抗体。结果①表达、纯化的MBPhEra,相对分子质量Mr为78×103,其纯度为95%。②从抗血清中纯化获得可与MBPhEra特异性结合的兔抗hEra多克隆抗体。结论获得了特异性较好的纯化兔抗hEra多克隆抗体。Aim To purify rabbit anti hEra polyclonal antibody. Methods The recombinant fusion protein MBP hEra was expressed in the E.coli and was purified through amylose resin chromatography column and Superose 12 gel fitration.The purified MBP hEra was coupled to the NHS activated sepharoseTM to prepare affinity chromatography column to purify rabbit anti hEra polyclonal antibody. Results ①MBP hEra was expressed and purified successfully with relative molecolar mass(Mr)of 78×103and its purity could reach about 95%.② purified Rabbit anti hEra polyclonal antibody could bind specifically to MBP hEra. Conclusion The purified rabbit anti hEra polyclonal antibody has better specificity and purity.

关 键 词：人ERA 多克隆抗体 纯化 ERA基因 

分 类 号：R392－33[医药卫生—免疫学]