锰超氧化物歧化酶转染间充质干细胞对糖尿病足伤口的愈合作用  

The Wound Healing Effect of Manganese Superoxide Dismutase Transfecting Mesenchymal Stem Cells on Diabetic Foot

作　　者：纪亮[1] 张鹏[1] 沈雷[1] 

机构地区：[1]齐齐哈尔医学院解剖教研室,黑龙江齐齐哈尔161006

出　　处：《中外医疗》2014年第14期17-19,共3页China & Foreign Medical Treatment

基　　金：黑龙江省教育厅科学技术研究面上项目(No:12521635)

摘　　要：目的阐明锰超氧化物歧化酶(MnSOD)转染间充质干细胞(MSC)对糖尿病足(DF)的治疗作用和机制,为干细胞技术治疗疾病奠定研究基础。方法链脲佐菌素法建立C57BL/6J小鼠糖尿病足模型,分别以2×104 MSC的实验组(pcDNA3.1-MnSOD转染MSC)、空白质粒组(仅转染pcDNA3.1)和对照组(未转染者为MSC)进行局部注射治疗,在7、14 d观察伤口愈合情况,并分别切取皮肤组织,进行CD34免疫组化染色,观察血管密度变化,ELISA法检测各组观察点的匀浆皮肤组织中超氧化物歧化酶(SOD)、谷胱甘肽氧化还原酶(GSHPX)变化。结果与空白质粒组相比,MnSOD-MSC实验组伤口愈合明显加快,差异有统计学意义(P<0.01);MnSOD-MSC实验组血管密度比空白质粒组高,差异有统计学意义(P<0.05);MnSODMSC实验组GSHPX、SOD含量明显比空白质粒组表达增高,差异有统计学意义(P<0.01)1。结论 MnSOD转染MSC可以促进抗氧化酶类表达增高,促进血管内皮细胞抗自由基损伤,加速血管新生和糖尿病足愈合。Objective To illustrate the therapeutic effect and the mechanism of manganese superoxide dismutase（MnSOD） transfecting mesenchymal stem cells（MSC） on diabetic foot（DF）, so as to lay a foundation for the research on diseases treated by stem cells. Methods Streptozotocin was used to establish C57BL/6J mice DF model, and local injection was done respectively to the experimental group of 2×104MSC（pcDNA3.1-MnSOD transfecting MSC）, the blank plasmid group（transfecting pcDNA3.1 only） and the control group（the un-transfected was MSC）. The wound healing was observed on the 7th and 14th day. Skin tissues were cut respectively for CD34 immunohistochemical staining, and the vascular density change was observed. ELISA was used to detect the change of superoxide dismutase（SOD） and glutathione oxidoreductase（GSHPX） in the homogenate skin tissue of each observation point. Results Compared with the blank plasmid group, wound healing in the MnSOD-MSC experimental group was accelerated obviously, P〈0.01; vascular density in the MnSOD-MSC experimental group was higher than that of the blank plasmid group, P 0.05; in the MnSOD-MSC experimental group, expression of content of GSHPX and SOD was significantly higher than that of the blank plasmid group, P〈0.01. Conclusion MnSOD transfecting MSC can promote the increase of antioxidant enzymes expression, promote vascular endothelial cells against free radical damage, and accelerate angiogenesis and healing of diabetic foot.

关 键 词：锰超氧化物歧化酶 间充质干细胞 自由基 糖尿病足 血管 

分 类 号：R730.2[医药卫生—肿瘤]