食管鳞癌患者血清Stathmin的表达及临床意义  

Clinical significance of serum Stathmin in esophageal squamous cell carcinoma

作　　者：赵楠[1] 韩波[1] 吴宗勇[2] 周兰萍[1] 刘芳[1] 颜露[1] 赵晓航[1,3] 许杨[1] 

机构地区：[1]中国医学科学院北京协和医学院肿瘤研究所分子肿瘤学国家重点实验室,北京市100021 [2]中国医学科学院北京协和医学院肿瘤医院检验科,北京市100021 [3]中国人民解放军海军总医院,北京市100037

出　　处：《世界华人消化杂志》2014年第14期2016-2022,共7页World Chinese Journal of Digestology

基　　金：国家自然科学基金资助项目;Nos.81372385;81071811;国家高技术发展计划项目"863"专题基金资助项目;Nos.2012AA02A503;2012AA020206~~

摘　　要：目的:探讨血清Stathmin的表达水平对食管鳞状细胞癌(esophageal squamous cell carcinoma,ESCC)的诊断价值及临床意义.方法:采用酶联免疫吸附法(enzyme-linkedimmunosorbent assay,ELISA)检测74例ESCC与81例健康对照血清Stathmin含量;采用ELISA检测34例食管鳞癌与40例健康对照血清角蛋白19片段抗原21-1(cytokeratin 19 fragment antigen 21-1,Cyfra21-1)的水平;采用化学发光法检测血清癌胚抗原(carcinoembryonic antigen,CEA)、癌抗原19-9(cancer antigens19-9,CA19-9)、癌抗原72-4(cancer antigens7 2-4,C A 7 2-4)水平;统计学分析血清中Stathmin浓度与临床参数的相关性.结果:ESCC组血清Stathmin水平显著高于健康对照组(P<0.001),ESCC组血清Stathmin水平与伴有淋巴结转移相关(P=0.036),血清Stathmin水平与患者性别、年龄、分化程度、TNM分期和肿瘤大小无显著相关.比较Stathmin和Cyfra21-1的受试者工作特性曲线(receiver operating characteristic curve,ROC曲线),提示Stathmin的灵敏度和特异性均优于Cyfra21-1,血清Stathmin水平对ESCC的诊断灵敏度可达94.6%.结论:血清Stathmin升高在食管鳞癌的发生发展中起重要作用,食管癌患者血清的Stathmin检测,对ESCC的诊断和预后判定具有一定意义.Stathmin可作为食管鳞癌诊断、治疗和判断预后的一个新的血清标志物,辅助临床诊断.AIM： To detect serum Stathmin in patients with esophageal squamous cell carcinoma（ESCC） and evaluate its correlation with clinicopathological parameters in ESCC.METHODS： Serum levels of Stathmin were measured by enzyme-linked immunosorbent assay（ELISA） in 74 patients with ESCC and 81 healthy individuals. Serum levels of Cyfra21-1 were measured by ELISA, and those of CEA, CA19-9 and CA72-4 were measured by chemiluminescence.RESULTS： Serum levels of Stathmin in ESCC patients were significantly higher than those in healthy controls（P 0.001）. Serum levels of Stathmin were correlated with lymph node metastasis（P = 0.036）, but not with age, gender, tumor differentiation, TNM stage or tumor size. According to the ROC curve, the sensitivity and specificity of serum Stathmin were significantly higher than those of Cyfra21-1 for diagnosis of esophageal squamous cell carcinoma. The sensitivity of serum Stathmin for diagnosis of ESCC was 94.6%. CONCLUSION： Serum Stathmin levels increase in ESCC patients. Detection of serum Stathmin is helpful for diagnosis and prognostic evaluation of esophageal cancer. Stathmin may serve as a new potential serum tumor marker for clinical detection, therapy and prognostic evaluation of ESCC.

关 键 词：STATHMIN 食管鳞癌 酶联免疫吸附法 

分 类 号：R735.1[医药卫生—肿瘤]