Combined collision-induced dissociation and photo-selected reaction monitoring mass spectrometry modes for simultaneous analysis of coagulation factors and estrogens  

作　　者：Quentin Enjalbert Marion Girod Jérémy Jeudy Jordane Biarc Romain Simon Rodolphe Antoine Philippe Dugourd Jér?me Lemoine Arnaud Salvador 

机构地区：[1]Université Lyon 1,CNRS [2]Université de Lyon,69622 Villeurbanne cedex,France [3]Institut Lumière Matière,UMR5306,France [4]Institut des Sciences Analytiques,UMR 5280,France

出　　处：《Journal of Pharmaceutical Analysis》2014年第3期183-189,共7页药物分析学报（英文版）

摘　　要：Oral estrogens are directly associated with changes in plasma levels of coagulation proteins. Thus, the detection of any variation in protein concentrations due to estrogen contraceptives, by a simultaneous analysis of both coagulation proteins and estrogens, would be a very informative tool. In the present study, the merit of photo-selected reaction monitoring （SRM）, a new analytical tool, was evaluated towards estrogens detection in plasma. Then, SRM and photo-SRM detection modes were combined for the simultaneous analysis of estrogen molecules together with heparin co-factor and factor XIIa, two proteins involved in the coagulation cascade. This study shows that photo-SRM could open new multiplexed analytical routes.Oral estrogens are directly associated with changes in plasma levels of coagulation proteins. Thus, the detection of any variation in protein concentrations due to estrogen contraceptives, by a simultaneous analysis of both coagulation proteins and estrogens, would be a very informative tool. In the present study, the merit of photo-selected reaction monitoring （SRM）, a new analytical tool, was evaluated towards estrogens detection in plasma. Then, SRM and photo-SRM detection modes were combined for the simultaneous analysis of estrogen molecules together with heparin co-factor and factor XIIa, two proteins involved in the coagulation cascade. This study shows that photo-SRM could open new multiplexed analytical routes.

关 键 词：ESTROGEN Coagulation factorprotein METABOLITE Photo-dissociation fragmentation SRM 

分 类 号：R917[医药卫生—药物分析学]