肠炎沙门氏菌血清型特异性基因挖掘及生物信息学分析  

Bioinformatics analysis and mining serovar-specific genes of Salmonella enterica serovar Enteritidis

作　　者：余水静[1] 彭艳平[1] 邓扬悟[1] 郭燕华[1] 梁长利[1] 欧阳城添[1] 

机构地区：[1]江西理工大学资源与环境工程学院,江西赣州341000

出　　处：《江西理工大学学报》2014年第3期13-17,共5页Journal of Jiangxi University of Science and Technology

基　　金：国家自然科学基金资助项目(31360388)

摘　　要：肠炎沙门氏菌(Salmonella enterica serovar Enteritidis)能引起畜禽的胃肠炎以及食物中毒,其血清型鉴定较为复杂.为了挖掘肠炎沙门氏菌血清型特异性基因,以44个已测序沙门氏菌基因组构建数据库,利用肠炎沙门氏菌所有蛋白编码序列为查询序列,通过BLASTN比对,挖掘出6个肠炎沙门氏菌血清型特异性基因(SEN1382,SEN1383,SEN1388,SEN1936,SEN1945和SEN1959),其中4个基因编码噬菌体相关蛋白.同时,研究分析了肠炎沙门氏菌血清型特异性基因的理化性质、二级结构、三级结构、亚细胞定位、信号肽和跨膜区等特征,结果表明肠炎沙门氏菌血清型特异性基因具有多样性特征.挖掘的血清型特异性基因可为鉴定肠炎沙门氏菌提供检测靶标.Salmonella enterica serovar Enteritidis is one of the major causes of gastroententis anu Ioou poisoning in humans due to the consumption of poultry derivatives. To mine serovar-specific genes of S. enterica serovar Enteritidis, all CDSs of it were searched in a searching database of 44 other Salmonella genomic sequences using SMM-system tool. Six serovar-specific genes were identified including SEN1382, SEN1383, SEN1388, SEN1936, SEN1945, and SEN1959. Among them, four genes decoded phage-related proteins. The characteristics of these genes were analyzed including physicochemical properties, secondary structure, three-dimensional structure, signal peptide, transmembrane regions, and subcellular localization. The results indicated the diversification of these genes. These serovar-specific genes can be used as the targets for detecting and identifying S. enterica serovar Enteritidis.

关 键 词：肠炎沙门氏菌 血清型 特异性基因 生物信息学分析 

分 类 号：Q933[生物学—微生物学] R155.5[医药卫生—营养与食品卫生学]