链霉菌CA-1产磷脂酶D发酵条件的优化  

Optimization of fermentation conditions of phospholipase D from Streptomyces sp.CA-1

作　　者：韩海霞[1] 曹栋[1] 史苏佳[2] 王强[1] 

机构地区：[1]江南大学食品学院,江苏无锡214122 [2]江南大学理学院,江苏无锡214122

出　　处：《食品工业科技》2014年第13期176-180,共5页Science and Technology of Food Industry

基　　金："十二五"国家科技支撑计划(2012BAD33B05)

摘　　要：以链霉菌CA-1菌为出发菌株,通过单因素实验和正交实验,对该菌株发酵产磷脂酶D的条件进行优化。结果表明最佳产酶条件为:葡萄糖20g/L、牛肉膏8.75g/L+鱼粉蛋白胨8.75g/L、吐温20 32.5g/L、氯化钙2.5g/L,接种量1.5%、培养基初始pH7.0、培养温度30℃、培养时间3d,在最佳发酵条件下,酶活达到1605.9U/L,是优化前的7.72倍。The Streptomyces sp.CA-1 as purpose strain, by single-factor experiment and orthogonal experiment,the fermentation conditions for phospholipase D production were optimized. Results showed that the optimalfermentation conditions for producing enzyme, glucose 20g/L, beef extract 8.75g/L ＋ fishmeal peptone 8. 75g/L,twain 20 32.5g/L, calcium chloride 2.5g/L, inoculums 1.5%, medium initial pH 7.0, culture temperature 30℃,incubation time 3d.Under the best fermentation conditions,the enzyme activity could reach 1605.9U/L,which was7.72 times than that of unoptimized conditions.

关 键 词：磷脂酶D 产酶条件优化 链霉菌CA-1 

分 类 号：TS201.3[轻工技术与工程—食品科学]