超声波结合CMC-Na对马血中血红素提取工艺效果影响  

The impact of ultrasound combined with CMC-Na on the extraction of heme from horse blood

作　　者：代文婷[1] 陶永霞[1] 杨海燕[1] 白羽嘉[1] 冯作山[1] 

机构地区：[1]新疆农业大学食品科学与药学学院,新疆乌鲁木齐830052

出　　处：《食品工业科技》2014年第13期257-261,共5页Science and Technology of Food Industry

基　　金：新疆维吾尔自治区科技攻关计划(201130101-4)

摘　　要：以马血为试验材料,采用超声波与CMC-Na结合提取血红素,在单因素试验基础上,通过Box-Behnken设计和响应曲面法分析试验结果,建立数学模型,确定最佳提取条件。研究得出最佳工艺条件:超声功率为338W、超声时间为10min、去离子水的添加量是红细胞(RBC)体积的5倍、提取pH为5.5。此条件下,血红素产率达到最大值0.42g/50mL,与模型高度拟合。Heme was extracted from horse blood, using ultrasound method combined with CMC-Na. Based on thesingle factor experiment, the Box-Behnken design and response surface method were used to set up themathematical model and determine the best extraction conditions.As a result,the optimum process conditions wereobtained as followingultrasonic power was 338W, ultrasonic time was 10min,deionized water content was 5 timesthe size of red blood cells （RBC）, and extraction pH was 5.5. Under these conditions, the yield of heme reachedmaximum 0.42g/50mL which was in good agreement with the predicted value.

关 键 词：血红素 超声波 响应曲面法 

分 类 号：TS251.1[轻工技术与工程—农产品加工及贮藏工程]