印记基因SLC22A18的表达与乳腺癌侵袭能力的关系  

Relationship between the Expression of Imprinted Gene SLC22A18 and the Invasion of Human Breast Cancer

作　　者：贺黉裕[1] 张宏伟[2] 

机构地区：[1]复旦大学附属中山医院重症监护室,上海200032 [2]复旦大学附属中山医院普外科,上海200032

出　　处：《中国临床医学》2014年第2期134-136,共3页Chinese Journal of Clinical Medicine

摘　　要：目的:研究印记基因SLC22A18(solute carrier family 22,member 18)在乳腺癌中的表达情况及其与乳腺癌侵袭能力的关系。方法:采用Transwell方法评估2种不同恶性程度的乳腺癌细胞株MDA-MB-231(恶性程度高)和MCF-7(恶性程度低)的侵袭转移能力。分别采用实时荧光定量逆转录聚合酶链反应(RT-PCR)和蛋白质印迹法检测SLC22A18的mRNA和蛋白在这2种乳腺癌细胞株中的表达情况。结果:MDA-MB-231细胞株恶性程度高,穿过膜的细胞多,侵袭能力强;MCF-7细胞株恶性程度低,穿过膜的细胞少,侵袭能力弱;SLC22A18在MCF-7中的mRNA和蛋白表达水平高于MDA-MB-231;差异有统计学意义(P<0.01)。结论:印记基因SLC22A18的表达与乳腺癌细胞的侵袭能力相关,该基因有望作为一个抑癌基因抑制乳腺癌的转移。Objective：To assess the expression of imprinted gene SLC22A18 （solute carrier family 22, member 18）in human breast cancer and its relationship with the invasion of human breast cancer. Methods：Transwell assay was used to evaluate the invasion abilities of two breast cancer cell lines MDA-MB-231 （with high metastatic ability） and MCF-7 （with low metastatic ability）. Real time fluorescence quantitative reverse transcriptase-polymerase chain reaction and Western blotting was used to detect the mRNA and protein levels of SLC22A18 in the two breast cancer cell lines. Results： There were much more MDA- MB-231cells that passed through the Matrigel membrane than MCF-7. The invasion abilities of the two breast cancer cell lines were significantly different（P〈0.01）, mRNA and protein levels of SLC22A18 in MDA-MB-231 were significantly lower than those in MCF-7（P〈0.01 ）. Conclusions： SLC22A18 expression has correlation with the invasion abilities of human breast cancer cells. SLC22A18 may serve as a tumor suppressor gene which can restrain the invasion abilities of human breast cancer cells.

关 键 词：印记基因 SLC22A18 乳腺癌 侵袭力 

分 类 号：R737.9[医药卫生—肿瘤]