四氯乙烯胁迫对草鱼抗氧化酶活性的影响及其机理  

Effect of perchloroethylene on activities of antioxidant enzyme in grass carp and its mechanism

作　　者：王桂燕[1] 李锋[2] 史济月[1] 周启星[3] 

机构地区：[1]沈阳药科大学制药工程学院,辽宁沈阳110016 [2]沈阳大学师范学院,辽宁沈阳110044 [3]南开大学环境科学与工程学院,环境污染过程与基准教育部重点实验室,天津300071

出　　处：《中国环境科学》2014年第6期1579-1585,共7页China Environmental Science

基　　金：国家自然科学基金项目(21037002);辽宁省教育厅资助项目(2008715);辽宁省大学生创新训练计划资助项目(201310163016)

摘　　要：研究了四氯乙烯(PCE)对草鱼的肝胰脏、肾脏和鳃组织的超氧化物歧化酶(SOD)和过氧化物酶(POD)的影响.结果表明:草鱼肝胰脏SOD活性,在PCE低浓度胁迫时其SOD活性"先升后降"[72h SOD是(6665.84±106.87)U/g·FW,168h SOD是(3021.26±16.96)U/g·FW],高浓度胁迫时"先降后升再降"[24h SOD是(2175.16±185.14)U/g·FW,96h SOD是(5692.19±44.17)U/g·FW,168h SOD是(1297.70±11.52)U/g·FW];草鱼肾脏SOD活性在PCE所有浓度组胁迫均是"先降后升再降"的趋势;草鱼鳃组织SOD酶活性只有在PCE较低浓度短时间内没有显著变化,其它情况始终降低.在PCE低浓度和中等浓度胁迫时,草鱼肝胰脏POD活性始终降低,较低浓度和高浓度胁迫时肝胰脏POD活性"先降后升再降";草鱼肾脏POD活性在PCE低浓度胁迫时"先升后降再升又降"的趋势,而高浓度胁迫时"先降后升又降";PCE对草鱼鳃组织POD活性"先升后降"的趋势.但是草鱼鳃组织的SOD和POD活性明显低于肝胰脏和肾脏组织的SOD和POD活性.Effect of perchloroethylene（PCE） on superoxide dismutase systems（SOD） and peroxidase（POD） in grass carp＇s hepatopancreas, kidney and gill tissue was investigated. The result shows： the activity of SOD in hepatopancreas represent ‘increase and then decrease＇ [72h SOD was（6665.84±106.87）U/g·FW,168h SOD was（3021.26±16.96） U/g·FW] under the stress of PCE at low concentration and ‘increase then decrease and increase again＇ [24h SOD was（2175.16±185.14）U/g·FW, 96h SOD was（5692.19±44.17）U/g·FW,168h SOD was（1297.70±11.52） U/g·FW] under the stress of PCE at high concentration; the activity of SOD in kidney represent ‘decrease and then increase＇ at any concentration of PCE; the activity of SOD in gill tissue does not change much except at low concentration of PCE within a short time, the activity always decreases under other circumstances. The activity of POD in hepatopancreas always decreases under the stress of PCE at low and moderate concentration and ‘decreases then increases and decreases＇ at the stress of relatively low and high concentration of PCE. The activity of POD in kidney ‘increases, decreases, then increases and decreases＇ at low concentration of PCE and ‘decreases and increases then decreases＇ at high concentration. The results indicate that the activity of SOD and POD in grass carp＇s gill tissue is much lower than SOD and POD in hepatopancreas and kidney.

关 键 词：四氯乙烯(PCE) 草鱼(Ctenopharyngodon idellus) 超氧化物歧化酶(SOD) 过氧化物酶(POD) 

分 类 号：X171.5[环境科学与工程—环境科学]