解淀粉芽孢杆菌固体培养条件优化  

Study on optimal solid culture condition of Bacillus amyloliquefaciens

作　　者：孙镇平[1] 刘洪红[2] 王忠敏[1] 孙伟[2] 单哲[2] 李敬盼[2] 

机构地区：[1]扬州大学兽医学院,江苏扬州225009 [2]扬州大学动物科学与技术学院,江苏扬州225009

出　　处：《扬州大学学报（农业与生命科学版）》2014年第1期27-30,共4页Journal of Yangzhou University：Agricultural and Life Science Edition

基　　金：江苏省科技厅前瞻性高新技术研究项目(BY2011138);江苏省高校优势学科建设工程项目(2012-06)

摘　　要：解淀粉芽孢杆菌(Bacillus amyloliquefaciens,BA)固体发酵可以大幅度提高动物益生菌制剂产量,但BA发酵的培养条件限制了该技术的广泛应用。为掌握BA的生长繁殖特性,采用正交设计研究BA固体发酵的最适培养条件。结果表明:对BA菌落数的影响因素主次顺序为温度、料水比、菌液接种量和pH值。当温度为38℃、初始pH为7.2、料水比为1∶1.8、菌液接种量为15%时,BA固体培养生长较好,可以获得更多活菌数。其最适培养条件有利于益生菌工厂化生产。The solid fermentation of Bacillus amyloliquefaciens （BA） can significantly increase the yield of probioties, and the fermentation culture conditions of BA limits the extensive application of the technology. The optimum culture conditions of solid fermentation for BA bacteria were studied in this experiment by orthogonal testing in order to investi- gate the characteristics of growth and reproduction of bacteria BA. Results showed that the influencing factors to the bacteria were temperature, the ratio of material to water, microbial inoculation amount and pH respectively. On 38 ℃ temperature, initial pH 7.2, and water ratio 1 ： 1.8, microbial inoculation quantity was 15 %, BA tended to grow more rapidly on the condition of solid fermentation and a higher number of viable cells were obtained. The best condition is conductive to the industrial production of probiotics.

关 键 词：解淀粉芽孢杆菌 培养条件 活菌数 

分 类 号：R155.1[医药卫生—营养与食品卫生学]