微流体芯片技术检测食源性致病菌的应用研究  

Lab-on-chip for the detection of food borne pathogenic bacteria

作　　者：陈炯[1] 屠丽红[1] 陈艳新[1] 秦胜营[2] 

机构地区：[1]上海市疾病预防控制中心,上海200336 [2]上海交通大学Bio-X研究院,上海200030

出　　处：《上海预防医学》2014年第6期330-334,共5页Shanghai Journal of Preventive Medicine

基　　金：上海交通大学Bio-X研究院和新加坡威特实验室的支持

摘　　要：[目的]通过应用微流体芯片（lab-on-chip）技术检测食源性致病菌,包括霍乱弧菌、沙门菌、副溶血性弧菌、志贺菌等细菌的灵敏度和精确度,从而建立突发公共卫生事件现场实验室的解决方案。[方法]分别采用Lab-on-chip平台食源性病原体微流检测芯片和荧光PCR方法对食源性病原菌样本进行检测,对比分析食源性微流芯片检测方法的特异性、敏感度、准确性和重复性。根据Lab-on-chip说明书以及荧光定量PCP试剂盒操作说明进行操作。[结果]Lab-on-chip方法的霍乱弧菌、沙门菌、志贺菌、副溶血性弧菌检出限为5×10^3-10^3DNA Copies/mL。特异性、准确性和重复性都获得了良好的结果。[结论]Lab-on-chip方法具有快速、便捷、高效、准确等优点,可以在突发公共卫生事件中作为良好的检测工具和方法。Objective To set up an onsite protocol for abrupt emergencies of public health e-vents, with both the sensitivity and accuracy of the lab-on-chip（microfluidic chip）, for the detection of food borne pathogenic bacterium including Vibrio cholera , Salmonella , Vibrio parahaemolyticus and Shigellaare exanimated . [ Methods] By comparison of the results from the chips and fluorescence quantitative PCR were analyzed the specificity , sensitivity, accuracy and repeatability of the Lab-on-chip. [ Results] Acceptable specificity , accuracy and repeatability of the chips had been well achieved , the detection limit of the chips for the Vibrio cholera , Salmonella, Vibrio parahaemolyticus and Shigella was 5 ×10^3-10^3 DNA Copies/mL. [ Conclusion] Lab-on-chip is believed to be a fast , convenient , efficient tool with accept-able accuracy for public health emergencies .

关 键 词：微流体芯片 食源性致病菌 检测方法 

分 类 号：R155.5[医药卫生—营养与食品卫生学]