耐酸耐热普鲁兰酶菌株的筛选及发酵条件的研究  

SELECTION OF THE ACID AND HEAT-RESISTANT PULLULANASE-PRODUCING STRAIN AND ITS FERMENTATION CONDITIONS

作　　者：唐宝英[1] 朱晓慧[1] 刘佳[1] 

机构地区：[1]江苏省微生物研究所,无锡214063

出　　处：《微生物学通报》2001年第1期39-43,共5页Microbiology China

摘　　要：从土壤中分离出一株产普鲁兰酶的菌株 ,初步鉴定为芽孢杆菌 ,通过紫外及 EMS多次诱变及筛选 ,酶活从 1.6 u/ m L提高到 5.4 u/ m L。在此基础上对产酶条件进行了优化 ,酶活有了较大提高 ,最高产酶水平达 8.8u/ m L。初步研究了酶的性质 ,酶反应的最适温度和 p H分别为 75℃和 4 .6 ,在 55℃反应条件下 ,酶在p H4 .0～ 8.0范围内活性稳定。A strain producing pullulanase was isolated from soil and mutated by UV and EMS with the increase of pullulanase activity from 1.6 to 5.4 u/mL. The fermentation conditions and medium were studied and optimized. The enzyme activity can reach 8.8 u/mL under the optimal fermentation conditions and medium. The optimal pH and temperature for enzyme are 4.6 and 75℃ respectively. It shows a high stabiliby during pH4.0～8.0 at 55℃.

关 键 词：普鲁兰酶 芽孢杆菌 诱变 产酶条件 性质 

分 类 号：TQ920.1[轻工技术与工程—发酵工程]