对水稻第4号染色体长臂近端粒区一个过氧化物酶基因簇的结构分析  

Structural Analysis of a Gene Cluster Encoding Two Cationic and Three Anionic Peroxidases from Rice Chromosome 4

作　　者：陈泽华[1] 周波[1] 韩斌[1] 钱跃民 洪国藩[1] 

机构地区：[1]中国科学院国家基因研究中心,上海200233

出　　处：《生物化学与生物物理学报》2001年第2期163-172,共10页

摘　　要：通过对定位BAC克隆 q30 37(H0 2 0 7F0 1)的序列测定和分析 ,在其中一个 2 2 .5kb的区域发现一个由 5个第三类过氧化物酶基因 (依次命名为osp1、osp2、osp3、osp4、osp5 )组成的基因簇。分析表明 ,osp1、osp2和osp3分别含 1个内含子 ,osp4和osp5分别含 2个内含子。该 5个基因分别编码 338、335、336、343和 346个氨基酸残基的蛋白质 ,而且都具有N端信号肽序列 ,其中OSP1、OSP4、OSP5为阴离子过氧化物酶 ,OSP2、OSP3为阳离子过氧化物酶。对 5个基因间的两两比较分析和进化分析结果表明 :该基因簇是通过一系列的串联基因复制事件而形成 ;osp5与来自玉米的ap1和来自大麦 (Hordeumvulgare)的prx7为潜在的直向同源基因 ,而且 ,osp1 5与ap1。Sequence analysis of a rice BAC q3037(H0207F01) identified a cluster of five tandemly arranged peroxidase genes, osp1, osp2, osp3, osp4 and osp5 , within a 22.5 kb region. osp4, osp5 each have three exons interrupted by two introns, while osp1, osp2 and osp3 each have two exons interrupted by a single intron. The five genes were predicted products of 338, 335, 336, 343 and 346 amino acid residues, respectively, including putative signal peptide sequence at the amino termini. And OSP1, OSP4 and OSP5 were predicted to be anionic peroxidase, OSP2 and OSP3 are cationic. Comparative analysis and evolutionary analysis of the clustered genes and other peroxidase family members revealed that the gene cluster occurred by tandemly gene duplications(from osp5 to osp1 ); and that osp5, ap1 and prx7 were potential orthologies, and osp1 5, ap1 and prx7 constituted a novel evolutionary branch of class III peroxidases.

关 键 词：过氧化物酶 基因簇 串联基因复制 直向同源基因 水稻 染色体 

分 类 号：Q943.2[生物学—植物学]