肝癌中高表达基因fup1的克隆及其功能  

Cloning and Characterization of fup1, A Gene Highly Expressed in Hepatocellular Carcinoma

作　　者：潘巍[1] 席全胜[1] 夏双络 赵慕钧[1] 甘人宝[1] 李载平[1] 

机构地区：[1]中国科学院上海生命科学研究院生物化学与细胞生物学研究所,上海200031

出　　处：《生物化学与生物物理学报》2001年第2期173-178,共6页

基　　金：中国科学院应用研究与发展重大项目课题资助&&

摘　　要：原发性肝细胞癌是我国高发的恶性肿瘤之一 ,开展肝癌相关基因的研究具有重要的意义。从已经获得的在肝癌和正常肝对照中表达量有明显差异的EST片段入手 ,克隆了一个功能未报道的而可能与肝癌相关的基因 ,暂命名为 fup1。该基因编码区全长 12 33bp ,其产物的分子量约为 46kD ,等电点为 5 .48,可能是一个核蛋白。Northern印迹结果表明该基因在人类除心脏以外的多种正常组织中表达量很小 ,说明其分布具有一定的组织特异性。将整合有该基因的真核表达载体转染NIH 3T3细胞后 。Primary hepatocellular carcinoma(HCC) is one of the common malignant tumors in China. In our previous work, a gene named fup 1(function unknown protein 1) was isolated that was expressed differently in HCC and in normal liver. We assumed that it might be a candidate oncogene for the HCC. The fup 1 gene had a ORF of 1 233 bp, encoding a protein with M r of 46 kD and isoelectric point of 5.48. The sequence characteristics showed its possible localization in nuclei. Northern blots showed that this gene was weakly expressed in many types of human tissues, except in the heart, implying its tissue specific expression pattern. MTT assay of the NIH 3T3 cells transfected with this gene in the form of recombinant eukaryotic expression plasmid showed its enhancing role to cellular proliferation.

关 键 词：肝癌 fup1 克隆 组织特异性 转染 fup1基因 

分 类 号：R735.7[医药卫生—肿瘤]