脂肪酶在微乳液和微乳液凝胶中催化辛酸辛醇的酯化反应  

Esterification of Octanoic Acid with 1-octanol Catalyzed by Lipase in WO Microemulsions and in Microemulsion\|based Organogels

作　　者：周国伟[1] 黄锡荣[1] 李越中[2] 李干佐[1] 胡玮[2] 

机构地区：[1]山东大学胶体与界面化学教育部重点实验室,济南250100 [2]山东大学微生物技术国家重点实验室,济南250100

出　　处：《生物工程学报》2001年第2期224-227,共4页Chinese Journal of Biotechnology

基　　金：国家自然科学基金!资助项目 (2 990 30 0 6 )

摘　　要：The esterification reactions of octanoic acid with 1\|octanol catalyzed by Candida lypolytical (CL) lipase was studied in water\|in\|oil microemulsions formed by water/bis\|(2\|ethylhexyl)sulfosuccinate sodium(AOT)/isooctane.The results of kinetic study showed that the reaction follows a Ping\|Pong Bi\|Bi mechanism.The values of apparent kinetic parameters were determined.Lipase has also been immobilized in gelatin\|containing AOT microemulsion\|based organogels (MBGs) for retention of catalytic activity.These lipase\|containing MBGs proved to be a solid\|phase catalysts for use in apolar organic solvents,retaining its higher activity after many runs of esterification reactions.The esterification reactions of octanoic acid with 1\|octanol catalyzed by Candida lypolytical (CL) lipase was studied in water\|in\|oil microemulsions formed by water/bis\|(2\|ethylhexyl)sulfosuccinate sodium(AOT)/isooctane.The results of kinetic study showed that the reaction follows a Ping\|Pong Bi\|Bi mechanism.The values of apparent kinetic parameters were determined.Lipase has also been immobilized in gelatin\|containing AOT microemulsion\|based organogels (MBGs) for retention of catalytic activity.These lipase\|containing MBGs proved to be a solid\|phase catalysts for use in apolar organic solvents,retaining its higher activity after many runs of esterification reactions.

关 键 词：油包水微乳液 酶催化 固定化酶 微乳液凝胶 动力学 辛酸醇 酯化反应 

分 类 号：O621.25[理学—有机化学] [理学—化学] O648.12