Class I patatin基因5′侧翼区驱动的GUS基因在马铃薯块茎中专一性表达  

1.4 kb 5′ FLANKING REGION OF CLASS I PATATIN DIRECTS TUBER-SPECIFIC GUS EXPRESSION IN POTATO (SOLANUM TUBEROSUM L.)

作　　者：宋东光[1] 周健[1] 黄大庆[1] 马欢[1] 司徒继锋[1] 王光清[1] 汪训明[1] 

机构地区：[1]复旦大学遗传学研究所遗传学和遗传工程国家重点实验室,上海200433

出　　处：《实验生物学报》2001年第1期5-10,共6页Acta Biologiae Experimentalis Sinica

基　　金：国家"863"高科技计划;国家自然科学基金(39700092);上海市高校科技发展基金~~

摘　　要：将1.4kb Class I patatin基因的5′侧翼区与GUS基因融合,构建了双元表达载体pPATIs(含patatin部分信号顺序)和pPATI(不含patatin部分信号顺序)。pPATI通过基因枪介导在块茎切片中获得了瞬间表达。以上建构物通过农杆菌介导转入了马铃薯品种Desiree。X-Gluc染色(PATIs不能染色)及PCR结果证实已获得转基因植株。利用离体块茎诱导系统,GUS的表达进一步用荧光进行定量检测,结果显示,PATI-GUS的转基因植株中GUS比活性均以块茎明显高于茎段,达10-20倍。蔗糖浓度的升高,PATI-GUS植株中的GUS比活性无明显变化,与前人报道有不同。此外,光照促进PATI-GUS的表达。Binary vectors pPATIs (with partial signal sequence) and pPATI (without signal sequence) were constructed by fusing 1.4 kb 5 flanking regions of Class I patatin gene with GUS. Transient GUS expression was observed in in vitro tuber slices bombarded with pPATI. These constructs were then introduced into potato (cv. Desiree) via Agrobacterium tumefaciens transformation. Trans-genie potato plants were confirmed by X-Gluc stainingand PCR. Using in vitro tuberization system, GUS activities were assayed by fluorescence. It was shown that, in plants transformed with PATI-GUS, GUS specific aci-tivites were about 10 - 20 fold higher in tubers than in stems. Increased sucrose concentration could not induce PATI-GUS expression, but light enhanced PATI-GUS expreasion in cultured shoots.

关 键 词：马铃薯 块茎 表达 CLASS I patatin基因 GUS基因 

分 类 号：S532[农业科学—作物学] Q943.2[生物学—植物学]