云南种植玛咖不同部分化学成分和抗氧化活性分析  

Chemical Composition and Antioxidant Activity Analysis of Different Parts of Maca Cultivated in Yunnan

作　　者：张维 张铁 王伟伟 王国宇 王斌 李晓军 

机构地区：[1]杭州艺福堂茶业有限公司研发中心,杭州310052

出　　处：《天然产物研究与开发》2014年第6期813-818,823,共7页Natural Product Research and Development

基　　金：杭州市农业发展基金(97);杭州市"雏鹰计划"(20121831K0155)

摘　　要：以云南丽江产玛咖植物的可食用部分根茎部分和地上部分为原料,通过强酸水解,邻苯二甲醛(OPA)和9-芴甲基氯甲酸酯(FMOC)柱前衍生法测定氨基酸含量,测试了两部分醇提后的氯仿萃取液中生物总碱的含量,并对该萃取液的抗氧化活性进行了研究。结果表明:根茎部分的氨基酸构成与地上部分种类相似,根茎部分的氨基酸含量高于地上部分,总氨基酸含量,根茎部分为15.10%,地上部分为14.29%,然而从营养评价上来说,Thr、Ile、Lys、Leu在CS和AAS两种评分体系下地上部分优于根茎部分,并且差异极显著(P<0.01),地上部分EAAI指数都优于根茎部分;两个部分都含有较高的生物碱成分,根茎部分为7.39 mg/g,地上部分为3.65mg/g,都具有清除DPPH·和·OH的能力,但同绿茶相比处于较低水平。该研究为玛咖可食用的根茎部分营养评价提供了依据,为地上部分的营养价值提供了数据基础,为玛咖的生物活性机制研究提供了参考。The purpose of this study was to determine the protein,alkaloids content in the roots and aerial parts of Maca cultivated in Lijiang,Yunnan. Constituents and content of protein in both parts was analyzed by hydrolyzing in HCl and pre-column derivatization using OPA and FMOC as auxiliary reagents. Both parts weresuccessively extracted by ethanol and chloroform. Thealkaloids content and radical scavenging capacity of the extract was studied. The amino acid composition of both parts was similar. Total amino acid content in roots （ 15. 10% ） was higher than that of aerial parts （14. 29% ）. However, Scores of Thr, lie, Lys, Leuin FAO/WHO and Egg system showed that nutritive value of aerial parts was significant higher than that rcots（P 〈0.01 ）. The chloroform extract of both roots and acrial parts showed radicals （ DPPH · and · OH） scavenging capacity, and had no significant correlation with content of alkaloids. The content of alkaloids in roots and aerial parts was 7.39 mg/gand 3.65 mg/g,the radicals scavenging capacity of both parts were much lower than that of green tea. This work will be a base for development of antioxidative functional food for acrial parts of Macaas well as for the further investigation on the mechanism of biological efficacy of Maca.

关 键 词：玛咖 地上部分 营养评价 生物碱 抗氧化活性 

分 类 号：R284.1[医药卫生—中药学]