耐盐紫甘薯花色苷组分分析和抗氧化性研究  

Composition Analysis and Antioxidant Activity of Anthocyanins from Salt-tolerant Ipomoes batatas L.

作　　者：孙梦茹[1] 朱大伟[1] 姚忠[2] 王明丽[1] 

机构地区：[1]常州市第一人民医院,常州213003 [2]南京工业大学食品与轻工学院,南京210009

出　　处：《天然产物研究与开发》2014年第6期935-942,共8页Natural Product Research and Development

基　　金：国家科技支撑计划课题项目(2012BAD34B08)

摘　　要：利用高效液相色谱与二极管阵列检测器/电喷雾质谱联用技术研究了耐盐紫甘薯Z103中的花色苷类化合物,确定该品种含有15种花色苷,主要为被咖啡酸、阿魏酸、对羟基苯甲酸等芳香酸酰化的矢车菊苷和芍药苷,其中矢车菊素3-O-对羟基苯甲酰-槐糖苷-5-O-葡糖苷、芍药素3-O-对羟基苯甲酰-槐糖苷-5-O-葡糖苷、芍药素3-O-阿魏酰-槐糖苷-5-O-葡糖苷为首次报道;同时进一步考察了不同体系中,紫甘薯花色苷抑制脂质过氧化能力和对DPPH·、O-·2和HO·的清除作用,结果表明紫甘薯花色苷具有较强的抗氧化能力,且均具有量效关系。紫甘薯花色苷(0.4 mg/mL)对脂质体氧化的抑制率为83.24%,对DPPH·(0.20 mg/mL)、O-·2(4 mg/mL)和HO·(30μg/mL)的清除率分别为94.06%、96.62%和96.12%。In this study,the composition of anthocyanins in salt-tolerant lpomoes batatas L. （purple sweet potato） Z103 were analyzed by high performance liquid chromatography （HPLC） coupled with a DAD detector and electrospray ionization tandem mass spectrometry （ESI-MS/MS）. In total, 15 kinds of anthocyanins were detetced：maiuly were cyanidin and peonidin derivatives which were acylated by caffeic acid, fendic acid, p-hydroxybenzoic acid and other aromatic acid. 3 kinds of anthecyanins：eyaniding 3-O-hydroxybenzoyl-sophoroside-5-O-glueoside, peonidin 3-O-hydroxybenzoyl - sophoroside-5-O-glucoside,peonidin 3-O-feruloyl-sophoroside-5-O-glueoside were detected from this plant for the first time. In addition, four assays, including liposome peroxidation assay, DPPH · scavenging assay, pyrogallol autoxidation assay and Fenton reactions ,were used to compare the antloxldant activity of salt-tolerant purple sweet potato Z103. The results showed that anthocyanins from purple sweet potato （APSP） had high antioxidant activity in a dose-dependent manner. The inhibition rate of APSP （0.4 mg/mL） in liposome peroxidation system was 83.24% and DPPH . scavenge rate of APSP （0.2 mg/mL） was 94.06% ,the O2^＊ scavenging rate of APSP （4 mg/mL） was 96.62% ,and the HO · scavenging rate reached 96.12% when the dosage of APSP was 30 μg/mL.

关 键 词：紫甘薯 花色苷 组分分析 抗氧化性 

分 类 号：R93[医药卫生—生药学] Q946.836[医药卫生—药学]