弱化F_1F_0-ATPase植物乳杆菌的选育及产酸性能分析  

Screening and Physiological Characteristics Analysis of Lactobacillus plantarum with Reduced F_1F_0-ATPase

作　　者：张庆[1,2] 钟小廷[1,2] 李京 林凯[1,2] 袁春红[1,2] 宋菲菲[1,2] 向文良[1,2] 车振明[1] 

机构地区：[1]西华大学生物工程学院,四川省食品生物技术重点实验室,四川成都610039 [2]西华大学古法发酵(酿造)生物技术研究所,四川成都610039 [3]中国检验认证集团四川有限公司,四川成都610063

出　　处：《食品与发酵科技》2014年第3期21-25,共5页Food and Fermentation Science & Technology

基　　金：四川省食品生物技术重点实验室科研项目(Szjj2013-048);优质中国泡菜现代产业链关键技术研究集成与产业化示范(2012NZ0002-8)

摘　　要：以植物乳杆菌为研究对象,利用硫酸新霉素筛选压力,筛选获得三株F1F0-ATPase活性弱化突变株M04、M15和M20。产酸及耐酸性能分析表明:突变株M04、M15和M20的产酸性能均弱于出发株,其中M20的产酸性能明显下降;在pH5.5和4.5的MRS液体培养基中M20的生长明显受到抑制;编码F1F0-ATPase的β亚基碱基序列分析表明:三株突变株均发生碱基突变而弱化F1F0-ATPase活性,其中突变株M20在多个位点的碱基置换及缺失对菌株的F1F0-ATPase活性影响最大。Three mutant strains of Lactobacillus plantarum M04, M15 and M20 with reduced F1F0-ATPase activity were screened from acid production degeneration of Lactobacillus plantarum in MRS medium containing 500μg/mL neomycin sulfate. The characteristics of acid production results showed that the parent strain was better than three mutant strains of M04, M15 and M20. The ability of acid production of M20 declined significantly. The tolerance of acid results showed that the growth of mutant M20 was inhibited significantly in the pH 4.5 or pH5.5 of MRS liquid medium. The base sequence encoded β subunit of F1F0-ATPase of three mutant strains and original strain re-vealed that the site mutation have effect on the F1F0-ATPase activity. Among them, the F1F0-ATPase activity of mutant M20 changed greatly.

关 键 词：植物乳杆菌 硫酸新霉素 F1F0-ATPase 突变株筛选 

分 类 号：TS252.54[轻工技术与工程—农产品加工及贮藏工程]