Effect of Zhuyeshigao granule on tumor necrosis factor-α and inter leukins serum protein levels in rats with radiation esophagitis  

作　　者：Junzhang Lu Chen Wang Xiutang Cao Minghui Yang Hong Zhao Yi Liu 

机构地区：[1]Department of Traditional Chinese Medicine, Chinese People's Liberation Army General Hospital [2]Department of Traditional Chinese Medicine,Department of Medical Statistics, Chinese People's Liberation Army General Hospital

出　　处：《Journal of Traditional Chinese Medicine》2014年第3期338-341,共4页中医杂志（英文版）

基　　金：Supported by National Natural Science Fund of China(Effect of Modified Zhuyeshigao granule and Its Componentson Preventing Radiation Esophagitis of Rats)(No.81173195);Capital Medical Development Fund(Traditional Chinese Medicine Class,No.SF-2009-III-45)

摘　　要：OBJECTIVE： To investigate the effects of Zhuyesh- igao granule （ZSG） on tumor necrosis factor-a （TNF-a）, interleukin-1 （IL-1）, IL-2, IL-6, and IL-8 in rats with radiation esophagitis. METHODS： Fifty Wistar rats were randomly divid- ed into five groups （10 rats in each group）： con- trol （without radiation）, saline-treated, and low, medium, and high-dose ISG-treated groups. Rats were given normal saline （10 mL/kg） or 1.15, 2.3, or 4.6 g/kg ZSG by intragastric administration once a day for 7 days. A rat model of radiation esophagi- tis was established by local irradiation of Co60 （490.25 cGy/min, totaling 30 Gy）. The administra- tion of ZSG was continued for another 7 days and on the 7th day post-irradiation, inferior vena cavablood was collected. The serum was separated, and TNF-a, IL-1, IL-2, 11_-6, and IL-8 protein levels were determined. RESULTS： Inflammatory response factors were found in the serum of each group. However, levels in ZSG-treated groups were significantly lower than in the saline-treated group （P〈0.05）. CONCLUSION： ZSG may prevent the development of radiation esophagitis, perhaps by inhibiting the generation and release of the inflammatory re- sponse factors TNF-a, IL-1, IL-2, IL-6, and IL-8.OBJECTIVE: To investigate the effects of Zhuyeshigao granule(ZSG) on tumor necrosis factor-α(TNF-α), interleukin-1(IL-1), IL-2, IL-6, and IL-8 in rats with radiation esophagitis.METHODS: Fifty Wistar rats were randomly divided into five groups(10 rats in each group): control(without radiation), saline-treated, and low,medium, and high-dose ISG-treated groups. Rats were given normal saline(10 mL/kg) or 1.15, 2.3,or 4.6 g/kg ZSG by intragastric administration once a day for 7 days. A rat model of radiation esophagitis was established by local irradiation of Co60(490.25 cGy/min, totaling 30 Gy). The administration of ZSG was continued for another 7 days and on the 7th day post-irradiation, inferior vena cava blood was collected.The serum was separated, and TNF-α, IL-1, IL-2, IL-6, and IL-8 protein levels were determined.RESULTS: Inflammatory response factors were found in the serum of each group. However, levels in ZSG-treated groups were significantly lower than inthesaline-treatedgroup(P<0.05).CONCLUSION: ZSG may prevent the development of radiation esophagitis, perhaps by inhibiting the generation and release of the inflammatory responsefactorsTNF-α,IL-1,IL-2,IL-6,andIL-8.

关 键 词：Zhuyeshigao granule ESOPHAGITIS Radi-ation injuries  experimental Tumor necrosis fac-tor-alpha INTERLEUKIN-1 INTERLEUKIN-2 INTERLEUKIN-6 INTERLEUKIN-8 

分 类 号：R273[医药卫生—中西医结合]