P2X家族受体在急性T淋巴细胞白血病小鼠腹腔巨噬细胞中的表达  

Expression of P2X Family Receptors in Peritoneal Macrophages of Mouse with Acute T Lymphoblastic Leukemia

作　　者：陈莎燕 冯文利 杨骁 廖金凤 王丽娜 林永敏 任倩 郑国光 

机构地区：[1]中国医学科学院、北京协和医学院,血液学研究所、血液病医院实验血液学国家重点实验室,天津300020

出　　处：《中国实验血液学杂志》2014年第3期623-628,共6页Journal of Experimental Hematology

基　　金：国家自然科学基金(81370634,81090412和81170511);北京协和医学院研究生创新基金(2012-1001-022);天津市自然科学基金(11JCZDJC18200);教育部新世纪优秀人才支持计划(NCET-08-0329)

摘　　要：本研究旨在探讨Notch1诱导的小鼠急性T淋巴细胞白血病模型中,腹腔巨噬细胞P2X家族受体的表达变化规律及其与巨噬细胞活化状态的相关性。建立小鼠急性T淋巴细胞白血病模型后,根据F4/80和CD206两个表面标记,用流式细胞术分别分选腹腔巨噬细胞以及CD206+的M2样和CD206-的M1样腹腔巨噬细胞;用实时定量PCR法研究各亚群细胞中P2X家族受体的表达变化规律。结果表明:腹腔巨噬细胞、M2样和M1样腹腔巨噬细胞均表达除P2X5受体外的其他P2X家族受体,且表达强度中等。各受体的表达随白血病发展变化而有所不同,其中腹腔巨噬细胞中P2X1和P2X7受体的表达逐渐提高,P2X2和P2X3受体表达在白血病晚期显著降低,P2X4受体表达仅在中期有所降低,P2X6受体表达无显著变化。在白血病发展中期M2样和M1样腹腔巨噬细胞间P2X家族受体表达亦存在差异,其中M2样巨噬细胞P2X1受体的表达水平显著低于M1样巨噬细胞,而M2样巨噬细胞P2X7受体表达水平显著高于M1样巨噬细胞,提示P2X家族受体表达与腹腔巨噬细胞活化状态相关。结论:白血病小鼠腹腔巨噬细胞P2X家族受体表达与白血病进程和巨噬细胞活化状态相关。本研究为深入探究巨噬细胞在白血病发展中的作用机制奠定基础。This study was aimed at exploring the expression pattern of P2X family receptors （P2XR） in peritoneal macrophages and their relationship with the activation states of macrophages in Notchl-induced mouse T-ALL model. After establishment of the leukemia model, F4/80 ＋ peritoneal macrophages, F4/80 ＋ CD206＋ M2-1ike and F4/80 ＋ CD206 - M1- like peritoneal macrophages were sorted by flow cytometry based on F4/80 and CD206 surface markers. The expression of P2XR in each cell population was detected by real time RT-PCR. The results showed that macrophages, Ml-like and M2- like macrophages moderately expressed P2XR except for P2X5R. The expression of P2XR varied with the development of leukemia. The expression of P2X1R and P2X7R in peritoneal macrophages increased steadily; the expression of P2X2R and P2X3R decreased at late stage of leukemia;the expression of P2X4R slightly decreased at intermediate stage;the expression of P2X6R kept unchanged. At intermediate stage of leukemia, the expression of P2XR in Ml-like and M2-1ike peritoneal macrophages varied. Ml-like macrophages expressed higher level of P2X1R than M2-1ike macrophages, whereas M2-1ike macrophages expressed higher level of P2X7R than Ml-like macrophages, which suggested that the expression of P2XR were related to the activation states. It is concluded that the expression of P2XR in peritoneal macrophages from leukemia mice is related to the progression of leukemia and the activation states of macrophages, which lay a foundation for further studying the role of macrophages in the development of leukemia.

关 键 词：急性T淋巴细胞白血病 腹腔巨噬细胞 P2X家族受体 活化状态 NOTCH1 

分 类 号：R733.71[医药卫生—肿瘤]