高脂饮食对1，2-二甲基肼诱导大鼠肠道肿瘤的影响  

Effect of high-fat diet on 1,2-dimethylhydrazine-induced colon tumors

作　　者：伍雯[1] 朱庆超[1] 杨永志[2] 石忱长[1] 高仁元[1] 秦环龙[2] 

机构地区：[1]上海交通大学附属第六人民医院普外科,200233 [2]上海市第十人民医院普外科,200072

出　　处：《中华临床营养杂志》2014年第3期171-177,共7页Chinese Journal of Clinical Nutrition

基　　金：国家自然科学基金（81230057）;国家高技术研究发展计划（863计划）（SS2014AA020803）;上海市市级医院新兴前沿技术联合攻关项目（SHDC12012106）

摘　　要：目的用1，2-二甲基肼诱导大鼠肠道肿瘤，同时观察高脂对1，2-二甲基肼诱导大肠肿瘤的影响。方法Westar大鼠50只，鼠龄7周，根据不同饮食及是否腹腔注射二甲基肼将大鼠分为4组：标准饮食对照组（n=10）、标准饮食±二甲肼诱导成瘤（SDT，n=15）组、高脂饮食对照组（n=10）、高脂饮食±二甲肼诱导成瘤（HFDT，n=15）组。实验18周后处死大鼠，酶联免疫吸附法检测大鼠血清甘油三酯（TG）、肿瘤坏死因子（TNF）-α及结肠TNF-α、白介素-6（IL-6）水平；取出肠道，观察肿瘤部位、数量、大小等，制作病理切片，观察组织学改变，免疫组织化学法检测TNF-α、Ki-67在正常组织、肿瘤中的分布。结果实验结束时SDT组大鼠死亡率20．00％、成瘤率75．00％、荷瘤率117％，HFDT组大鼠死亡率26．67％、成瘤率81．82％、荷瘤率191％，SDT组和HFDT组死亡率、成瘤率（P=0．545）及荷瘤率（χ^2=1．343，P=0．247）差异均无统计学意义。SDT组中肿瘤平均体积〉0．05cm^3个数占28．57％，HFDT组中肿瘤平均体积〉0．05cm^2个数占66．67％，两组肿瘤平均体积差异具有统计学意义（P=0．030）。高脂饮食大鼠血清TG[（1．39±0．31）mmol／L]、TNF-α[（124．80±21．69）ng／L]较标准饮食大鼠血清TG[（0．46±0．20）mmol／L]、TNF-α[（85．83±17．45）ng／L]显著升高（P值均=0．000）。高脂饮食大鼠结肠黏膜TNF-α[（6．22±0．63）ng／g]、IL-6[（13．50±0．67）ng／g]较标准饮食大鼠结肠黏膜TNF-α[（2．33±0．44）ng／g]、IL-6[（7．31±0．41）ng／g]显著升高（P=0．020，P=0．000）；高脂饮食对照组Ki-67阳性表达率40％，较标准饮食对照组10％显著升高（P=0．028）；HFDT组Ki-67阳性表达率90．48％较SDT组50％显著升高（P=0．015）。结论高脂饮食可能通过导致大鼠血清TG、TNF-α上升，上调结肠黏膜炎症因子TNF-α、IL-6及增殖细胞�Objective To explore the effect of fat on 1,2-dimethylhydrazine （DMH） -induced colon tumors. Methods A total of 50 7-week-old male Wistar rats were further divided into four groups： standard diet feed control group （ n = 10）, standard diet feed plus DMH-induced tumor group （ SDT, n = 15 ）, high-fat diet feed control group （ n = 10） and high-fat diet feed plus DMH-induced tumor group （ HFDT, n = 15 ）. Rats were killed 18 weeks later, and enzyme-linked immunosorbent assay was used to detect serum triglyceride, tumor necrosis factor （TNF-ot）, and colonic TNF-α, interleukin-6. After the intestinal tracts were removed, the location, amount, and size of the tumors were observed. The pathological changes of the tissue sections were observed, and the distributions of TNF-α and Ki-67 in the normal tissues and tumors were detected by immunohistochemistry. Results Upon the completion of the study, the mortality rate of rats was 20.00% in the SDT group and 26. 67% in the HFDT group, the tumor formation rate was 75.00% in the SDT group and 81.82% in the HFDT group, and the tumor-bearing rate was 117% in the SDT group and 191% in the HFDT group. No statistical significance difference between the two groups in mortality rate, tumor formation rate （ P = 0. 545 ） and tumor bearing rate （χ^2 = 1. 343, P = 0. 247 ）. The average tumor volume was significantly different between the standard diet feed control group and high-fat diet feed control group （28.57% vs 66. 67% , P = 0. 030）. Also, the serum triglyceride and TNF-α levels significantly differed between the SDT group and HFDT group [TG （ 1.39 ±0. 31 ） mmol/L and TNF-α （ 124. 80 ±21.69） ng/L in the HFDT group and TG （0. 46 ±0. 20） mmol/L and TNF-α （85.83 ± 17.45） ng/L in the SDT group] （P =0. 000）. The expressions of TNF-α, IL-6, and Ki-67 in colonic mucosa were significantly higher in the high-fat diet feed control group than in the standard diet feed control group [ TNF-α： （6. 22 ±0. 63） ng/g vs （2.

关 键 词：高脂饮食 1 2-二甲基肼 大肠癌 

分 类 号：R735.3[医药卫生—肿瘤]