响应面法优化小丛红景天多糖超声提取工艺研究  

Optimization of ultrasonic-assisted extraction of polysaccharides from Rhodiola dumulosa(Franch.) S.H.F. by response surface analysis

作　　者：王莉[1] 赵桦[1] 马英丽[1] 王星[1] 

机构地区：[1]陕西理工学院生物科学与工程学院,陕西汉中723000

出　　处：《食品工业科技》2014年第14期315-320,共6页Science and Technology of Food Industry

基　　金：陕西省教育厅专项科研计划项目(12JK0842);陕西省重点学科专项建设经费资助(2013)

摘　　要：为优化小丛红景天药材中水溶性多糖超声提取工艺,在单因素实验的基础上,利用响应面分析方法建立了小丛红景天多糖超声提取的二次响应曲面方程。实验证实提取温度、超声功率、提取时间和料液比对小丛红景天多糖提取有不同的影响。结果表明,在实验范围内通过方差分析可知各因素对小丛红景天多糖提取率影响的大小依次为超声提取温度、超声功率、料液比和超声提取时间。小丛红景天多糖超声提取工艺的最佳条件为:提取温度69℃、超声功率240W、料液比1∶30(g/mL),提取时间39min,在此条件下小丛红景天多糖提取率可达4.305%,与模型预测值高度相符。In order to optimize the conditions of ultrasonic-assisted extraction method of polysaccharides from Rhodiola dumulosa（Franch.） S.H.F., on the base of single-factor experiment, an equation for the ultrasonic wave extraction was built. The experimence showed that extraction temperature,ultrasonic power,extraction time and liquid-solid ratio had different effects on the extraction yield of polysaccharides. The result indicated that within tested level ranges,the importance of four process parameters affecting extraction yield of polysaccharides order was ultrasonic treatment temperature,ultrasonic power,solid-liquid ratio and ultrasonic treatment time. The optimum conditions of the ultrasonic wave extraction of R, durnulosa polysaccharides were as follows .. temperature 69℃,extraction ultrasonic power 240W,solid-liquid ratio 1：30（g/mL） and extraction time 3gmin,the extraction rate of R. dumulosa polysaccharides could be up to 4.305% ,exhibiting a good agreement with the predicted values.

关 键 词：小丛红景天 多糖 超声提取 响应面分析法 

分 类 号：TS201.1[轻工技术与工程—食品科学]