叠氮溴化丙锭对食品中单增李斯特氏菌检测结果的影响  

Influence of propidium monoazide( PMA) on detection of Listeria monocytogenes in food

作　　者：周阳[1] 罗岚[2] 唐泰山[2,3] 沈涛[2] 徐幸莲[3] 郭云昌 叶可萍[3] 付瑞燕[1] 祝长青[2,3] 

机构地区：[1]安徽农业大学茶与食品科技学院,安徽省食品安全分析与检测省级实验室,安徽合肥230000 [2]江苏出入境检验检疫局,江苏南京210001 [3]南京农业大学,江苏南京210095 [4]国家食品安全风险评估中心,北京100022

出　　处：《江苏农业学报》2014年第3期640-644,共5页Jiangsu Journal of Agricultural Sciences

基　　金：国家质检总局课题(2012IK168);农业部行业科技项目(200903012);农业部产业体系项目(CARS36-11)

摘　　要：为研究叠氮溴化丙锭(PMA)在单增李斯特氏菌检测中的作用,利用实时荧光PCR方法检测不同浓度PMA处理的单增李斯特氏菌标准菌株CMCC 54004的死菌和活菌核酸的扩增情况。结果显示,1.0 mg/ml PMA能有效抑制单增李斯特氏菌死菌核酸的扩增。经过PMA处理后单增李斯特氏菌死菌与未经PMA处理的检测结果间差异极显著,而PMA处理后CMCC 54004活菌与未经PMA处理的检测结果间差异性不显著。通过对PMA处理的CMCC 54004死菌、活菌、不同比例死菌与活菌混合液以及其在不同食品基质下的检测结果的比较,进一步证实,1.0 mg/ml PMA在单增李斯特氏菌检测中能有效减少死菌核酸的影响,提高检测结果的可靠性。In this study, real-time fluorescence PCR method was employed to detect the nucleic acid amplification of dead cells and living cells of Listeria monocytogenes standard strain CMCC 54004 treated with different concentrations of propidium monoazide（ PMA） . The results showed that 1. 0 mg/ml PMA could effectively inhibit nucleic acid amplifica-tion in dead bacteria. There was a significant difference between the test results of Listeria monocytogenes dead bacteria treated with PMA and without PMA, however, no obvious difference was seen after PMA treatment on CMCC 54004 living bacteria. Further researches on CMCC 54004 dead cells, living cells and proportionally mixed dead and liv-ing cells treated with PMA and PMA in the presence of food interference suggested that 1. 0 mg/ml PMA could effectively reduce the influence of dead bacteria nucleic acid in Listeria monocytogenes detection, leading to relia-ble test results.

关 键 词：单增李斯特氏菌 叠氮溴化丙锭( PMA) 

分 类 号：TS201.6[轻工技术与工程—食品科学]