复合纤维素降解菌固体发酵培养基的筛选及优化  

The Optimization of Solid-state Fermentation Culture Medium for Combined Cellulose Degradation Bacteria

作　　者：张立霞[1] 李艳玲[1] 屠焰[1] 刁其玉[1] 

机构地区：[1]中国农业科学院饲料研究所,农业部饲料生物技术重点开放实验室,北京100081

出　　处：《中国畜牧杂志》2014年第13期77-82,共6页Chinese Journal of Animal Science

基　　金：秸秆饲料生物转化技术研究与示范(20120304202)

摘　　要：旨在提高筛选的复合菌黄孢原毛平革菌、黑曲霉、绿色木霉、青霉的滤纸酶活(FPA),制成干粉应用于农作物秸秆的降解,提高其降解粗饲料所含纤维素、半纤维素、木质素等的能力。采用单因素试验对该培养基的组成成分和发酵条件进行筛选;利用Placket-Burman对培养基的组成成分进行重要性排序,选择可信度>85%的因素进行后续试验;采用响应面分析方法,对筛选得到的的培养基成分进行优化,得到纤维素酶活最高时各因素的水平。结果表明:最适碳源和最适氮源分别是麸皮和豆粕;P-B试验筛选了3种培养基成分豆粕、(NH4)2SO4、蛋白胨;响应面分析优化后发酵培养基的成分为麸皮20 g、豆粕8.97 g与营养液等比例搅拌均匀;优化后的发酵培养基产纤维素酶活达到0.2621 U/mL,比优化前提高了。培养条件为接种量8%,培养温度32℃,发酵时间为5 d。To improve the enzymatic activity of the combination of Phaerochaete chrysosporium, AspergiUus Niger, Trichoderma viride , peniciUium, one-factor-at-a-time approach was used screen the composition of the medium and fermentation conditions; Using the response surface design of experiment to optimize the composition of the culture medium, it got the highest level of the cellulose enzyme activity at the time. Single factor test results showed that the carbon source and the nitrogen source was respectively bran and soybean meal; The response surface analysis determined the composition of the fermentation culture medium. The results showed that the cellulose enzyme activity of optimized fermentation reached 0. 291 U/mL.

关 键 词：纤维素酶 固态发酵 发酵培养基 响应面法 培养基优化 

分 类 号：S814.2[农业科学—畜牧学]