虎纹蛙消化道淀粉酶和脂肪酶活力研究  

A Study on the Activities of Amylase and Lipase in Digestive Tract of Rana rugulosa

作　　者：李庆[1] 张雪[1] 余昌萍 汪娟[1] 余晶晶[1] 张盛周[1] 

机构地区：[1]安徽师范大学生命科学学院生物环境与生态安全省级重点实验室,安徽芜湖241000

出　　处：《激光生物学报》2014年第2期159-164,共6页Acta Laser Biology Sinica

基　　金：安徽省自然科学基金(NO.11040606M75);国家级大学生校外实践教育基地大学生创新训练计划项目;安徽师范大学本科生优秀毕业论文培育计划项目(NO.pyjh2013195);安徽省重要生物资源保护与利用重点实验室建设基金

摘　　要：以酶学分析方法研究了虎纹蛙消化道淀粉酶和脂肪酶的分布以及pH和温度对这两种消化酶活力的影响。结果表明:在各自生理pH值条件下,虎纹蛙消化道不同部位淀粉酶活力大小顺序依次为前肠>中肠>后肠>食道>胃,胃和肠淀粉酶最适pH值分别为8.6和7.0,最适温度分别为35℃和40℃。脂肪酶活力大小顺序依次为中肠>后肠>前肠>胃>食道,各部位之间差异显著(P<0.05),胃和肠脂肪酶的最适pH值均为9.0,最适温度分别为50℃和55℃。The distribution of amylase and lipase in the digestive tract of Rana rugulosa and the effects of pH and tem perature on the activities of these digestive enzymes were studied by enzymatic methods. The results indicated that the activities of digestive enzymes in different parts of digestive tract were different significantly. The activities of amylase from high to low were the foregut, the midgut, the hindgut, the esophagus and the stomach. The optimum pH values of the amylase in stomach and intestine were 8.6 and 7.0 respectively, and their optimum temperature were 35 ℃ and 40 ℃ respectively. The activities of lipase from high to low were the midgut, the hindgut, the foregut, the stomach and the esophagus, respectively. The differences between various parts of digestive tract were significant （ P 〈 0.05 ）. The optimum pH values of the lipase in stomach and intestine were equally 9.0, and their optimum temperature were 50 ℃ and 55 ℃ respectively.

关 键 词：虎纹蛙 淀粉酶 脂肪酶 最适PH值 最适温度 

分 类 号：Q55[生物学—生物化学]